8453 UV-visible system - operator manual.pdf

Good Measurement PracticesGeneral Considerationsa solvent for the analyte, and on experimental conditions. Table 1 listscommon solvents and the lower limit of their useful wavelength range.Table 1Lower Limit of UV Transmission for Some Common SolventsLower LimitSolvent180–195 nm Sulfuric acid (96%)WaterAcetonitrile200–210 nm Cyclopentanen-HexaneGlycerol2,2,4-TrimethylpentaneMethanol210–220 nm n-Butyl alcoholIsopropyl alcoholCyclohexaneEthyl ether245–260 nm ChloroformEthyl acetateMethyl formate265–275 nm Carbon tetrachlorideDimethyl sulfoxideDimethyl formamideAcetic acid280–290 nm BenzeneToluenem-XyleneAbove 300 nmPyridineAcetoneCarbon disulfideWARNINGMany of the solvents in Table 1 are hazardous. Be sure you fullyunderstand their properties before using them.When using volatile solvents such as acetone or methylene chloride, makesure that the sample cell is stoppered. Evaporation of a solvent can change48