8453 UV-visible system - operator manual.pdf

Good Measurement PracticesGeneral ConsiderationsThe design of the flow cell should minimize entrapment of bubbles and flowchanneling to provide the most reliable results.Handling and Maintaining CellsPassivating New CellsWhen filling a non-passivated new cell with your sample, you will observethat air bubbles stick on the windows of your cell. To prevent the formationof sticky bubbles, rinse the cell with cleaning and passivating fluid (partnumber 5062-8529). The cleaning procedure you should apply is described onthe label of the cleaning fluid container.Cleaning CellsThe fats in fingerprints are significant absorbers in the UV region and, if lefton optical surfaces, can cause erroneous results. Wipe off all fingerprints andcontaminants before using a sample cell.Use only high quality lens tissues (part number 9300-0761) and never drythe inside of a cell with lens tissues. Dry the inside of the cell withpressurized, oil free air, that prevents the cell from getting contaminated withtissue particles, or rinse the cell with blank or sample solution. Floatingparticles in the cell will deflect the light beam and so lead to a very poorquality of the measured spectrum.Figure 7Floating Particles in a CellFloating particles will deflectand scatter the light beam46