POLARIS GC/MS

New Finnigan Polaris GC/MS/MS ultimate selectivity for target analytes
Ion trap GC/MS/MS is the most
powerful chromatography technique a
scientist can utilize. MS/MS is quite
simply this:
A molecule is ionized in the normal
manner and the ions are sent into the
ion trap. The ion trap is tuned to a
single ion mode in which a preselected
(parent) ion is captured and
all other ions are allowed to pass
through the detector. The parent ion
is now energized within the ion trap
to dissociate it into a spectrum of
product ions. These unique product
ions are free from chemical
interference, which allows for extreme
sensitivity and detectivity in
complicated matrices.
High throughput is necessary in
today’s laboratory environment.
Difficult analyses are not exempt
from this requirement. Determination
of pesticides in food, endocrine
disrupters or dioxins in the
environment, drugs in biological fluids
and many others are all applications
that can be performed routinely and
accurately with GC/MS/MS.
Triazolam – Tricyclic Antidepressant
100
0
150
100
203.1
238.1
279.1
316.1
342.0
207.1 239.1 281.1
177.2
240.1 307.1
343.9
176.1
253.1
317.1
163.1
202.1 237.1
267.1
282.2
341.1
344.9
361.1
386.0
0
200 250 300 350 400
m/z
313.1 277.1
100
315.1
100
0
150
313.1
200 250 300 350 400
m/z
16.0 16.5 17.0 17.5 18.0 18.5 19.0 19.5 20.0 20.5 21.0 21.5 22.0
100
80
60
40
20
0
(a)
19.92
20.01
20.23
0
150
174.1
242.2
243.2
278.1
201.2
214.2 251.2 286.2
313.1
313.8
200 250 300 350 400
m/z
The process of MS/MS involves (a) ionization of the compound of interest; (b) isolation of a single ion; and
(c) dissociation of the isolated ion into its product ions. This process increases the selectivity of the analysis by
removing the matrix from the product ions.
Pesticides in Orange Extract – Matrix Removal
Full Scan
EI/MS
MS/MS Chromatogram
MS/MS of Fensulfothion
(parent ion - m/z 293)
156.0
158.1
(b)
Time (min)
21.33
21.35
100 120 140 160 180 200 220 240 260 280
m/ z
Isolation
Ionization Dissociation
The selectivity of MS/MS analysis is far superior to full scan EI or SIM. This allows the chromatographer to
achieve lower detection limits and unambiguous compound identification in the most difficult matrices.
19.91
217.9
236.0
237.0
(c)
263.9
265.0
265.9
293.0
300