Chromosome - Fapesp
Chromosome - Fapesp
Chromosome - Fapesp
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The long right homology arm (RHA) preferentially (always) directs integration<br />
dcd<br />
ACE and Insertion at PyrE<br />
catP<br />
Left homology<br />
arm (LHA)<br />
pMTL-JH12 ACE Vector<br />
[ Tm R ]<br />
REP<br />
Right homology<br />
arm (RHA)<br />
CAC0026 pyrE<br />
CAC0028<br />
BioBricks<br />
pyrE’ 4 3 2 1<br />
CAC0028<br />
− ACE plasmid replicon (REP) is defective: integrants grow faster on<br />
thiamphenicol<br />
<strong>Chromosome</strong><br />
[ Tm R FOA S Ura + ]<br />
homologous<br />
recombination
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