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Chromosome - Fapesp

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ACE and Insertion at PyrE<br />

A third iteration of the method can add further DNA (BioBricks 9-12)<br />

using the ACE vector pMTL-JH12<br />

BioBricks<br />

8 7 6 5<br />

dcd CAC0026 pyrE<br />

3 2 1 CAC<br />

BioBricks<br />

12 11 10 9<br />

dcd CAC0026 pyrE’<br />

CAC<br />

4<br />

BioBricks<br />

BioBricks<br />

8 7 6 5 4 3 2 1<br />

<strong>Chromosome</strong><br />

[ Tm S FOA S Ura + ]<br />

<strong>Chromosome</strong><br />

[ Tm S FOA R Ura - ]

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