Chromosome - Fapesp

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ACE Exemplification Iterative Exemplification of ACE: insertion of the entire lambda genome into the clostridial genome in 3 iterative cycles Success Confirmed by:- • Southern Blot • Nucleotide Sequencing An unprecedented achievement unmatched by any other group Heap et al (2012) Nucleic Acids Research. 2012 Jan 18.
ACE & Metabolic Engineering C. acetobutylicum ATCC 824 acetate acetone butyrate acetyl-CoA butyryl-CoA ethanol butanol John Heap
Page 1 and 2: BioEnergy Workshop, May 2012, São
Page 3 and 4: University of Nottingham UK Campus
Page 5 and 6: Multidisciplinary Centre Centre for
Page 7 and 8: Clostridia Research Group HEAD Prof
Page 9 and 10: http://www.bsbec.bbsrc.ac.uk Key fa
Page 11 and 12: Unlike Yeast Bacteria Utilise Pento
Page 13 and 14: Unlike Yeast Bacteria Utilise Pento
Page 15 and 16: S T R AI N I M PROVE M ENT Clostrid
Page 17 and 18: ACETONE Clostridia & Biofuels Bipha
Page 19 and 20: pH 7 6,5 6 5,5 5 4,5 4 3,5 Clostrid
Page 21 and 22: Clostridia & Biofuels ‘Systems Bi
Page 23 and 24: Clostridia & Biofuels ‘Systems Bi
Page 25 and 26: Streamlining the System http://www.
Page 27 and 28: Clostridia & Biofuels Metabolic Eng
Page 29 and 30: dcd ACE and Insertion at PyrE Inser
Page 31 and 32: The long right homology arm (RHA) p
Page 33 and 34: Double crossover selected on the ba
Page 35 and 36: ACE and Insertion at PyrE More BioB
Page 37 and 38: ACE and Insertion at PyrE The singl
Page 39 and 40: ACE and Insertion at PyrE The ACE p
Page 41: ACE and Insertion at PyrE A third i
Page 45 and 46: Removal of Co-products? � C. acet
Page 47 and 48: Removal of Co-products? � C. acet
Page 49 and 50: BB-2 prefix BB-2 suffix GAATTCGCGGC
Page 51 and 52: BB-2 prefix BB-2 scar BB-2 suffix P
Page 53 and 54: RBS ORF RBS ORF BioBrick-2 Assembly
Page 55 and 56: RBS RBS BioBrick-2 Assembly ctfA ct
Page 57 and 58: ACE & Metabolic Engineering Product
Page 59 and 60: ACE & Cellulosic Butanol Cellulosom
Page 61 and 62: Systems Biology of BioButanol Germa
Page 63 and 64: International Collaboration OTHER (

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