Biochemistry/Molecular Biology - ARVO

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ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Biochemistry/Molecular BiologyPurpose: To identify differences in the protein composition of theaqueous humor (AH) of patients with diabetic retinopathy (DR)compared to patients without history of diabetes undergoing cataractsurgery (control).Methods: AH samples (n=6 for control and n=7 for DR) wereanalyzed by Liquid Chromatography/Tandem Mass Spectrometry(LC-MS/MS) and relative protein abundances were determined byspectral counting. G test followed by post hoc Holm Sidak was usedfor statistical analyses to determine significance in the differentialexpression of proteins between DR and control groups. Proteins wereclassified into functional groups using Scaffold 3.0.Results: Using stringent filtering criteria, seven proteins weresignificantly altered in the DR AH (p
ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Biochemistry/Molecular BiologyAventix Animal Helth (F), Aventix Animal Helth (R), TherapeuticVision, Inc (F), Therapeutic Vision, Inc (F), Therapeutic Vision, Inc.(R), Threapeutic Vision, Inc (S), US 20090105269 (P)Support: NIH grant EY016730Program Number: 1160 Poster Board Number: D0064Presentation Time: 1:00 PM - 2:45 PMThe significant relationships between Fibronectin, Tenascin-Cand Periostin in the eyes of the patients with diabetic retinopathyYukio Sassa 1, 2 , Shigeo Yoshida 2 , Keijiro Ishikawa 2 , Ryo Asato 2 ,Mitsuru Arima 2 , Masanori Miyazaki 2 , Hiroshi Enaida 2 , Yuji Oshima 2 ,Toshihiro Kono 1 , Tatsuro Ishibashi 2 . 1 Ophthalmology, FukuokaUniversity Chikushi Hospital, Fukuoka, Japan; 2 Ophthalmology,Kyushu University, Fukuoka, Japan.Purpose: Periostin (PN) , Tenascine-C (TN) and Fibronectin (FN)are extracellular matrix (ECM)proteins, which compose fibrovascularmembrane (FMV) in ocular proliferative diseases. We havepreviously reported that PN expression increased in the vitreous ofpatients with proliferative diabetic retinopathy (PDR).We examinedthe correlations among TN, FN and PN in the vitreous of patientswith macular hole (MH), diabetic macular edema (DME) and PDR.Methods: Vitreous fluid was obtained from 198 individuals (MH of28 eyes in 28 individuals, DME of 20 eyes in 20 individuals, PDR of163eyes in 150 individuals) undergoing pars plana vitrectomy atFukuoka University Chikushi Hospital and Kyushu University (fromOctober 2007 to March 2011) in accordance with Human DiscardedSpecimen Research Protocol approved by institutional review boards.Undiluted samples were collected at the time of surgery, immediatelystored at -80 °C until used. The concentrations of PN/ TN/ FN weredetermined with ELISA method at the same time.Results: The concentrations of PN/ TN/ FN significantly increased inpatients with DME (average 1.48/ 10062/ 0.64 ng/ml) and PDR(average 12.16/ 4131/ 0.92 ng/ml) compared to those in MH (0.19/749/ 0.36 ng/ml) (p
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Biochemistry/Molecular Biology - ARVO

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