Biochemistry/Molecular Biology - ARVO

ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Biochemistry/Molecular Biologydelivered in BSA along with 100 µM PalmCoA and 30 µM CRALBPfor one hour in a 37C shaking water bath. Retinoids were extractedand analyzed by HPLC in comparison to retention times andauthentic retinoid standards.Results: Bovine RPE homogenates (chelator control) yielded 80pmol of 11cisRE/mg of protein when incubated with all-trans retinol.In the presence of 2,2’-bipyridne, the amount of 11-cisRE’s droppedto 38 pmol/mg of protein. Primary Chicken Müller cell homogenatesincubated with all-trans retinol yielded 116 pmol of 11-cisRE/mg ofprotein. Primary Chicken Müller cell homogenates that wereincubated with the iron chelator, 2,2’-bipyridine, yielded no 11-cisRE’s. Levels of 11-cis retinol remained constant at 24 pmol/mg ofprotein in homogenates incubated both with and without the additionof 2,2’-bypridine iron chelator.Conclusions: Isomerase 2 activity in Müller cells of the conedominatedchicken retina is iron-dependent.Commercial Relationships: Brandi S. Betts-Obregon, None;Andrew S. Mendiola, None; Andrew T. Tsin, NoneSupport: NIH SCORE Program Grant # GM08194, National Centerfor Research Resources (5G12RR013646-12) and the NationalInstitutes of Minority Health and Health Disparities (G12MD007591)Program Number: 3768 Poster Board Number: A0107Presentation Time: 2:45 PM - 4:30 PMSerum and Macular Response to Lutein Supplementation: ASystematic Review and Meta-AnalysisEstelle S. Lowry 2 , Christopher Cardwell 1 , Jayne Woodside 1 , Ruth E.Hogg 2 . 1 Centre for Public Health, Queens University Belfast, Belfast,United Kingdom; 2 Centre for Vision and Vascular Science, QueensUniversity Belfast, Belfast, United Kingdom.Purpose: To systematically review the evidence currently availableon the effects of lutein supplementation on serum lutein and macularpigment and investigate the concept of retinal nonresponse.Methods: Three databases were searched systematically to identifyhuman studies in English language, published up to February 2012.Studies were selected using pre-specified criteria. Of 77 potentialarticles identified, 24 articles met the selection criteria. Meta-analysisand meta-regression techniques were used to quantitatively poolresults and investigate the association between study characteristicsand mean changes in serum lutein and macular pigment (MP).Results: Intervention studies using lutein only and combined luteinsupplements were included. Daily dosage ranged from 2.4mg to30mg lutein and duration of the supplementation period varied from35 to 365 days. The review included 19 open label studies, 12placebo-controlled and 10 randomised, placebo-controlled studies.All studies reported an increase in mean serum lutein and all but tworeported an increase in MP levels. There was strong heterogeneityamongst studies. Dosage was found to be a significant influence inthe increase of serum lutein levels in healthy volunteers. For eachincrease of 10mg of lutein, there was an increase of 0.02µmol-1 (95%CI 0.003, 0.04) P