Biochemistry/Molecular Biology - ARVO

ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Biochemistry/Molecular BiologySupport: European Research Council ref: 281096.Clinical Trial: ISRCTN13894787Program Number: 3774 Poster Board Number: A0113Presentation Time: 2:45 PM - 4:30 PMLens status and macular pigment optical density measured by thetwo wavelength autofluorescence method: the Alienor StudyMarie B. Rougier 1 , Cecile Delcourt 2, 3 , Marie-Noelle Delyfer 1, 2 ,Melanie Le Goff 2, 3 , Florence Malet 1 , Jean Francois Dartigues 2, 3 ,Joseph Colin 1, 3 , Jean-Francois Korobelnik 1, 2 . 1 Service dOphtalmologie, CHU-Bordeaux Univ de Bordeaux, Bordeaux,France; 2 INSERM U897, Bordeaux, France; 3 Université Bordeaux2,Bordeaux, France.Purpose: To determine whether the lens status (lens opacities, lensextraction with PC IOL) is associated with differences in macularpigment optical density measured with the two wavelengthautofluorescence method.Methods: 963 residents of Bordeaux (France), aged 73 years ormore, were included in 2006-2008. About 18 months later, acomplementary eye examination was proposed to all subjectsdiagnosed with early ARM at baseline, and an equal number ofsubjects without early ARM. It included a measurement of macularpigment optical density (MPOD) using two wavelengthautofluorescence method (Modified HRA I, Heidelberg Engineering,Heidelberg, Germany). Lens opacities were graded at slit lampaccording to the LOCSII methodology. Nuclear cataract was definedas NO grade greater than 3 and/or NC grade greater than 3. Corticalcataract was defined as C grade greater or equal to 0.5. Posteriorsubcapsular cataract was defined as P grade greater or equal to 0.3.Among 395 included subjects, we excluded 66 subjects with late agerelatedmacular degeneration and 93 subjects with other maculardiseases (macular hole, retinal occlusion, myopic maculopathy,retinitis pigmentosa, laser scars, epiretinal membranes …) and 30subjects using dietary supplements containing lutein and zeaxanthin.Among the remaining 206 subjects, 181 subjects (321 eyes) hadcomplete data for lens opacities and macular pigment measurements.Associations of MPOD with lens status were estimated usingmultivariate mixed linear regression models, taking into account datafrom both eyes and their intra-individual correlations.Results: After adjustment for age and gender, MPOD within 0.5°was higher in eyes having undergone lens extraction ( +0.071 opticaldensity units, p=0.002) and lower in eyes with nuclear cataract (-0.19optical density units, p=0.0001), but was not different according tocortical or posterior subcapsular opacities. Similarly, MPOD with 1°was higher in eyes having undergone lens extraction (+0.069 opticaldensity units, p=0.001) and lower in eye with nuclear cataract (-0.15optical density units, p=0.001).Conclusions: Lens status appears to affect macular pigment opticaldensity measured using the two wavelength autofluorescence method.Commercial Relationships: Marie B. Rougier, THEA (C),Bausch&Lomb (C), Allergan (C), Kemin (C); Cecile Delcourt,Laboratoires Théa (F), Novartis (C), Bausch+Lomb (C); Marie-Noelle Delyfer, Thea Laboratories (F); Melanie Le Goff, None;Florence Malet, None; Jean Francois Dartigues, Novartis (F),IPSEN (F); Joseph Colin, Alcon (C), Abbott (C),AdditionTechnology (C); Jean-Francois Korobelnik, Alcon (C),Allergan (C), Bayer (C), Carl Zeiss Meditec (C), Novartis (C), Thea(F)Support: Financial support: Laboratoires THEAProgram Number: 3775 Poster Board Number: A0114Presentation Time: 2:45 PM - 4:30 PMThe relationship between visual performance and AMD severityin subjects with early age-related macular degenerationJohn M. Nolan 1 , Sarah Sabour-Pickett 1 , Jim Stack 1 , JamesLoughman 2 , Alan Howard 3 , Stephen Beatty 1 . 1 Macular PigmentResearch Group, Waterford Institute of Technology, Waterford,Ireland; 2 Optometry, Dublin Institute of Technology, Dublin, Ireland;3 Howard Foundation, Cambridge University, Cambridge, UnitedKingdom.Purpose: To investigate the relationship between measures of visualperformance (VP) (using a range of psychophysical parameters) andAMD severity in subjects with early age-related maculardegeneration (AMD) (ARERDS grade 2 to 8).Methods: Data was collected from 66 subjects with early AMD.Corrected distance visual acuity (CDVA) was measured using alogMAR chart. Contrast sensitivity (CS) and glare disability (GD)were measured using the sine wave grating-based Functional VisionAnalyser (FVA) . Retinotopic ocular sensitivity (ROS) wasmeasured by microperimetry. Stereo fundus images were graded forAMD status at the accredited reading centre at the University ofWisconsin.Results: There was a statistically significant inverse relationshipbetween measures of ROS and AMD-severity grade (p ≤ 0.01). Noother parameters of VP were significantly related to AMD severity (p> 0.05, for all).Conclusions: AMD status is related to ROS measurements, but is notrelated to other psychophysical measurements of VP, includingCDVA, in subjects with early AMD. This is an important finding, asCDVA is typically the only outcome measure used in the clinical andresearch setting for this condition.Commercial Relationships: John M. Nolan, None; Sarah Sabour-Pickett, None; Jim Stack, None; James Loughman, None; AlanHoward, None; Stephen Beatty, NoneSupport: Howard Foundation UKClinical Trial: ISRCTN81595685Program Number: 3776 Poster Board Number: A0115Presentation Time: 2:45 PM - 4:30 PMConcordance of macular pigment measurements usingheterochromatic flicker photometry, autofluorescence, andreflectometryJessica L. Dennison, Jim Stack, Stephen Beatty, John M. Nolan.Chemical & Life Sciences, Waterford Institute of Technology,Waterford, Ireland.Purpose: This study compares in vivo measurements of MP obtainedusing customized heterochromatic flicker photometry (cHFP)(Densitometer), dual-wavelength autofluorescence (HeidelbergSpectralis) and single-wavelength reflectance (Zeiss Visucam).Methods: MP was measured in one eye of 49 subjects using each ofthe three devices. Agreement between the Densitometer andSpectralis devices was investigated at various eccentricities (circa0.25, 0.50, 1.00, and 1.75 degrees eccentricity), using a variety ofquantitative and graphical methods (including Pearson correlation tomeasure degree of scatter (precision [prcn]), accuracy coefficient(acc) to measure shifts in location or scale, concordance correlationcoefficient (ccc) calculated from the acc and prcn coefficients, pairedt-test, and Bland-Altman plot). Because data at different eccentricitiesis not yielded from the Visucam, agreement between central MPusing the Visucam and each of the other devices was investigatedusing regression methods.Results: Agreement was strong between the Densitometer andSpectralis at all central eccentricities (e.g. at circa 0.25 degreeseccentricity: acc= 0.97, prcn= 0.90, ccc= 0.87). Regression analysisshowed a very weak relationship between the Densitometer and the©2013, Copyright by the Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Go to iovs.org to access the version of record. For permissionto reproduce any abstract, contact the ARVO Office at arvo@arvo.org.