Bacillus Genetic Stock Center Catalog of Strains Seventh Edition ...

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BACTERIOPHAGES BY ORIGINAL CODEOriginal CodeB1715V1Bace-11Bam35-cBam35-vBastilleBat 1Bat 10Bat 11Bat 18Bat 5Bat 7CP-54BerDP7GP-10mor1P400PK1Tb10TP-15BGSC No.1P291P251P261P271P301P311P341P351P361P321P331P371P381P391P401P421P411P431P2822/Back to Table of Contents
SELECTED CLONING VECTORS AND HOSTSBacillus thuringiensis plasmid-cured hostsBGSC No. Original Code Reference Description4D11 CRY(-)B Stahley DP, et al. (1978) BiochBiophys Res Comm 84:581Reported to be plasmid-cured strain of Bacillusthuringiensis subsp. kurstaki, but reacts with noknown Bt flagellar antisera4D22 HD-73-20 González J. et al. (1982) Proc NatlAcad Sci 79:6951Crystal minus derivative of Bacillus thuringiensissubsp. kurstaki HD-73; cured of 75, 11, and 10 kbplasmids4E5 4E3 Cry- Dean DH, unpublished Crystal minus derivative of Bacillus thuringiensissubsp. sotto 4E34Q7 4Q2-81 Clark BD (1987) Ph.D. Thesis (OhioSt. Univ)Bacillus thuringiensis subsp. israelensis plasmidcuredstrain4Q8 4Q2-81 str Dean DH (unpublished) Bacillus thuringiensis subsp. israelensis plasmidcuredstrain with chromosomal streptomycinresistance mutationOther Bacillus hostsBGSC No. Original Code Reference Description1A748 1012M15 (unpublished) B. subtilis host; restriction minus, sporulation plus;allows blue-white screening on X-gal with pHPS9;genotype glgB::lacZ∆M15 Km leu met r(-)m(+)1A751 MW10 Wolf M. et al. (1995) Microbiology141:281-2907A16 QMB1551 Quarter Master R&D Center, USArmy (unpublished)13A4 WHO2297 Davisdon E, donor; Abbott Labs,sourceB. subtilis host; deficient in major secretedproteases; genotype eglS∆102 bglT/bglS∆EV nprapr hisB. megaterium host; wild type isolateB. sphaericus host; wild type isolate; isolated inCeylon from Culex pipiens quinquefasciatusGram-positive plasmidsBGSC No. Original Code Reference Description1E6 BD366 Gryczan TJ, et al. (1978) JBacteriol 134:318B. subtilis host bearing pUB110; confers resistanceto kanamycin or neomycin and phleomycin; hostgenotype thr-5 trpC21E9 DSM402(pBC16)Kreft J, et al. (1978) Mol GenGenet 162:59B. subtilis host bearing pBC16; confers resistanceto tetracycline; host genotype trpC21E10 DSM402(pBC16-1)Kreft J, et al. (1978) Mol GenGenet 162:59B. subtilis host bearing pBC16-1; confers resistanceto tetracycline; host genotype trpC21E17 168(pC194) Erlich SD, source B. subtilis host bearing pC194; conferschloramphenicol resistance; host genotype trpC21E18 pE194 Weisblum B et al. (1979) JBacteriol 137:635B. subtilis host bearing pE194; conferserythromycin resistance; host genotype thr-5 trpC21E601012M15(pGVD1)Eijsink, unpublished (seeMolecular Biological Methodsfor Bacillus, Harwood &Cutting, eds., p 83)B. subtilis host bearing pGVD1; conferschloramphenicol resistance; 2571 bp plasmid withcopy number 150-200 in B. subtilis; contains multiplecloning site; host genotype glgB::lacZ∆M15 leu met7E2 PV311 Vary P (unpublished) B. megaterium host bearing pUB110; confersresistance to kanamycin or neomycin andphleomycin23/Back to Table of Contents
Page 1 and 2: Bacillus&thuringiensisBacilluscereu
Page 3 and 4: TABLE OF CONTENTSTable of Contents
Page 5 and 6: What you can do to help the BGSCOur
Page 7 and 8: Volunteer SpotlightClaire McHughCla
Page 9 and 10: Serotype 6—Serovar. entomocidus/s
Page 11 and 12: Serotype 17—Serovar. tohokuensisB
Page 13 and 14: Serotype 3a, 3d—Serovar. sumiyosh
Page 15 and 16: Serotype 45—Serovar. roskildiensi
Page 17 and 18: Serotype 18a, 18c—Serovar. yosooB
Page 19 and 20: B. THURINGIENSIS STRAINS BY SEROTYP
Page 21 and 22: E. COLI CLONES OF B. THURINGIENSIS
Page 23: BACTERIOPHAGES OF B. CEREUS & B. TH
Page 27 and 28: MEDIA FOR GROWTH AND SPORULATION2×
Page 29 and 30: G-Tris MediumAronson, A. I., et al.
Page 31 and 32: Electroporation ProtocolAdapted fro
Page 33 and 34: GENERALIZED TRANSDUCTION WITH CP-54
Page 35 and 36: References1. Andrup, L., J. Damgaar
Page 37 and 38: Purification of Crystals in a Separ
Page 39 and 40: Table 2. Sequences of the PCR prime
Page 41 and 42: NOMENCLATURE FOR CRY AND CYT PROTEI
Page 43 and 44: NAME ORIGINAL ACCESSION NUMBER(S) C
Page 45 and 46: 23. Donovan, W. P., J. M. González
Page 47 and 48: 66. Masson, L., A. Mazza, L. Gringo
Page 49 and 50: diptera-specific insecticidal endot
Page 51 and 52: NAME SOURCE STRAIN KNOWN TOXICITYCr
Page 53 and 54: Phylogram of Cry and Cyt Holotype S
Page 55 and 56: • What is the mode of action of C
Page 57 and 58: GENERAL INDEX OF STRAINS, PHAGE AND

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