Bacillus Genetic Stock Center Catalog of Strains Seventh Edition ...

G-Tris MediumAronson, A. I., et al. 1971. J. Bacteriol. 106:1016-1025.G-Tris is a general purpose growth and sporulation medium for B. cereus and B. thuringiensis.The following stocks are made up and sterilized in concentrated form, then mixed with sterile distilledwater to the following final concentrations:Tris-HCl (pH 7.6)0.01 MFeSO 4·7H 2 O 0.00005 %CuSO 4·5H 2 O 0.0005 %ZnSO 4·7H 2 O 0.0005 %MnSO 4·H 2 O 0.005 %MgSO 4 0.02 %CaCl 2·2H 2 O 0.008 %K 2 HPO 4 0.05 %(NH 4 ) 2 SO 4 0.2 %glucose, filter sterilized 0.1 %CDGS MediumNakata, H. M. (1964) J. Bacteriol. 88:1522.CDGS is a chemically defined growth and sporulation medium developed for B. cereus T. It is also usefulfor many B. thuringiensis strains, although the partial auxotrophic requirements can vary from strain tostrain.For CDGS, use the recipe for G-Tris with the following modifications:In the place of Tris-HCl (pH 7.6), use 0.1 M potassium phosphate buffer, pH 6.4.In the place of Yeast extract, use the following amino acid mixture to the following final concentrations:L-glutamic acid 1.84 µg/mlL-leucine 0.80 µg/mlL-valine 0.30 µg/mlL-threonine 0.168 µg/mlL-methionine 0.07 µg/mlL-histidine 0.05 µg/ml27/Back to Table of Contents