Bacillus Genetic Stock Center Catalog of Strains Seventh Edition ...

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Bacillus Genetic Stock Center Catalog of Strains, Seventh Edition,Part 2: Bacillus thuringiensis and Bacillus cereus© 1999Permission is given to copy this material or portions of it freely,provided that no alterations are made and that this copyright page is included.Daniel R. Zeigler, Ph.D.The Bacillus Genetic Stock CenterDepartment of BiochemistryThe Ohio State University484 West Twelfth AvenueColumbus, Ohio 43210Disclaimer: The information in this catalog is believed to be correct.Due to the dynamic nature of the scientific process and to normalhuman limitations in dealing with such a large amount of data,however, some undetected errors may persist. Users bear theresponsibility of verifying all important data before making a significantinvestment of time or other physical or financial resources.Cover: Scanning electron micrograph of an uncharacterized B.thuringiensis strain isolated on the Ohio State University campus.Spores and spherical crystals, covered with a mesh, are visible.Micrograph was digitized and converted to shadowed images withMacromedia Freehand ® 8.0.This material is based upon work supported by the National ScienceFoundation under Grant No. 9319712. Any opinions, findings, andconclusions or recommendations expressed in this material arethose of the author and do not necessarily reflect the views of theNational Science Foundation.
TABLE OF CONTENTSTable of Contents 1Obtaining Materials from the Bacillus Genetic Stock CenterWhat is the BGSC? 2What kinds of cultures are available from the BGSC? 2What you can do to help the BGSC 2How to order cultures 3Pricing information 3Important notice--please read before ordering! 4Bacillus thuringiensis StrainsBacillus thuringiensis strains indexed by BGSC accession number 5Bacillus thuringiensis strains indexed by original code 16Bacillus thuringiensis strains indexed by serotype 17Bacillus thuringiensis strains indexed by subspecies 18Escherichia coli Clones of Bacillus thuringiensis cry GenesEscherichia coli strains indexed by BGSC accession number 19Escherichia coli strains indexed by gene name 19Bacillus cereus Strains and Genomic LibraryBacillus cereus strains indexed by BGSC accession number and original code 20Bacillus cereus genomic library 20Bacteriophages of Bacillus cereus and Bacillus thuringiensisBacteriophages of B. thuringiensis and B. cereus indexed by BGSC number 21Bacteriophages indexed by original code 22Selected Cloning Vectors and HostsBacillus thuringiensis plasmid-cured hosts 23Other Bacillus hosts 23Gram positive plasmids 23Bacillus-E. coli shuttle vectors 24Antibiotic resistant cassettes 24Technical Material Relating to Bacillus thuringiensis and Bacillus cereusGrowth and sporulation media for Bacillus thuringiensis and Bacillus cereus 25Electroporation of Bacillus thuringiensis and Bacillus cereus 28Generalized transduction with CP-54Ber 31Conjugation-like mating with Bacillus thuringiensis 32Plasmid profiles Bacillus thuringiensis and Bacillus cereus 33Purification of Bacillus thuringiensis crystals 34Detection of cry and cyt genes by PCR 36Nomenclature of Cry and Cyt proteins 39Pesticidal activity of Cry and Cyt proteins 48The review literature for Bacillus thuringiensis 52Bacillus cereus and Bacillus thuringiensis--the species question 54General Index of Strains, Phage, and Plasmids 55
Page 1: Bacillus&thuringiensisBacilluscereu
Page 5 and 6: What you can do to help the BGSCOur
Page 7 and 8: Volunteer SpotlightClaire McHughCla
Page 9 and 10: Serotype 6—Serovar. entomocidus/s
Page 11 and 12: Serotype 17—Serovar. tohokuensisB
Page 13 and 14: Serotype 3a, 3d—Serovar. sumiyosh
Page 15 and 16: Serotype 45—Serovar. roskildiensi
Page 17 and 18: Serotype 18a, 18c—Serovar. yosooB
Page 19 and 20: B. THURINGIENSIS STRAINS BY SEROTYP
Page 21 and 22: E. COLI CLONES OF B. THURINGIENSIS
Page 23 and 24: BACTERIOPHAGES OF B. CEREUS & B. TH
Page 25 and 26: SELECTED CLONING VECTORS AND HOSTSB
Page 27 and 28: MEDIA FOR GROWTH AND SPORULATION2×
Page 29 and 30: G-Tris MediumAronson, A. I., et al.
Page 31 and 32: Electroporation ProtocolAdapted fro
Page 33 and 34: GENERALIZED TRANSDUCTION WITH CP-54
Page 35 and 36: References1. Andrup, L., J. Damgaar
Page 37 and 38: Purification of Crystals in a Separ
Page 39 and 40: Table 2. Sequences of the PCR prime
Page 41 and 42: NOMENCLATURE FOR CRY AND CYT PROTEI
Page 43 and 44: NAME ORIGINAL ACCESSION NUMBER(S) C
Page 45 and 46: 23. Donovan, W. P., J. M. González
Page 47 and 48: 66. Masson, L., A. Mazza, L. Gringo
Page 49 and 50: diptera-specific insecticidal endot
Page 51 and 52: NAME SOURCE STRAIN KNOWN TOXICITYCr
Page 53 and 54:
Phylogram of Cry and Cyt Holotype S
Page 55 and 56:
• What is the mode of action of C
Page 57 and 58:
GENERAL INDEX OF STRAINS, PHAGE AND
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