Bacillus Genetic Stock Center Catalog of Strains Seventh Edition ...

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NBY MediumThorne C. B. 1968. J. Virol. 2:657-662.Per liter of distilled water:Nutrient brothYeast extractAgar (if desired)8 g3 g15 gNBYS MediumLecadet, M.-M., M. O. Blondel, J. Ribier. 1980. J. Gen. Microbiol. 121:203-12.A complete medium for B. thuringiensis berliner and many other strains; useful for phage propagation.Per liter of distilled water:Nutrient brothYeast extractKClAgar (if desired)8 g3 g0.4 g15 gAutoclave. Cool to 55C and add from sterile stock solutions:1.2% MgSO 4·7H 2 O 1 ml0.1% MnSO 4·4H 2 O 1 ml0.014% FeSO 4·7H 2 O 1 ml7.5% CaCl 2·2H 2 O 1 mlPA MediumThorne C. B. 1968. J. Virol. 2:657-662.For assaying phage CP-54Ber and related phages. Gives 30% higher efficiency of plating that NBY.Per liter of distilled water:Nutrient brothNaClMgSO 4·7H 2 OAgar (if desired)8 g5 g0.2 g15 gAdjust pH to 5.9. Autoclave. Cool to 55C and add from sterile stock solutions:5% MnSO 4·4H 2 O 1 ml15% CaCl 2·2H 2 O 1 ml26/Back to Table of Contents
G-Tris MediumAronson, A. I., et al. 1971. J. Bacteriol. 106:1016-1025.G-Tris is a general purpose growth and sporulation medium for B. cereus and B. thuringiensis.The following stocks are made up and sterilized in concentrated form, then mixed with sterile distilledwater to the following final concentrations:Tris-HCl (pH 7.6)0.01 MFeSO 4·7H 2 O 0.00005 %CuSO 4·5H 2 O 0.0005 %ZnSO 4·7H 2 O 0.0005 %MnSO 4·H 2 O 0.005 %MgSO 4 0.02 %CaCl 2·2H 2 O 0.008 %K 2 HPO 4 0.05 %(NH 4 ) 2 SO 4 0.2 %glucose, filter sterilized 0.1 %CDGS MediumNakata, H. M. (1964) J. Bacteriol. 88:1522.CDGS is a chemically defined growth and sporulation medium developed for B. cereus T. It is also usefulfor many B. thuringiensis strains, although the partial auxotrophic requirements can vary from strain tostrain.For CDGS, use the recipe for G-Tris with the following modifications:In the place of Tris-HCl (pH 7.6), use 0.1 M potassium phosphate buffer, pH 6.4.In the place of Yeast extract, use the following amino acid mixture to the following final concentrations:L-glutamic acid 1.84 µg/mlL-leucine 0.80 µg/mlL-valine 0.30 µg/mlL-threonine 0.168 µg/mlL-methionine 0.07 µg/mlL-histidine 0.05 µg/ml27/Back to Table of Contents
Page 1 and 2: Bacillus&thuringiensisBacilluscereu
Page 3 and 4: TABLE OF CONTENTSTable of Contents
Page 5 and 6: What you can do to help the BGSCOur
Page 7 and 8: Volunteer SpotlightClaire McHughCla
Page 9 and 10: Serotype 6—Serovar. entomocidus/s
Page 11 and 12: Serotype 17—Serovar. tohokuensisB
Page 13 and 14: Serotype 3a, 3d—Serovar. sumiyosh
Page 15 and 16: Serotype 45—Serovar. roskildiensi
Page 17 and 18: Serotype 18a, 18c—Serovar. yosooB
Page 19 and 20: B. THURINGIENSIS STRAINS BY SEROTYP
Page 21 and 22: E. COLI CLONES OF B. THURINGIENSIS
Page 23 and 24: BACTERIOPHAGES OF B. CEREUS & B. TH
Page 25 and 26: SELECTED CLONING VECTORS AND HOSTSB
Page 27: MEDIA FOR GROWTH AND SPORULATION2×
Page 31 and 32: Electroporation ProtocolAdapted fro
Page 33 and 34: GENERALIZED TRANSDUCTION WITH CP-54
Page 35 and 36: References1. Andrup, L., J. Damgaar
Page 37 and 38: Purification of Crystals in a Separ
Page 39 and 40: Table 2. Sequences of the PCR prime
Page 41 and 42: NOMENCLATURE FOR CRY AND CYT PROTEI
Page 43 and 44: NAME ORIGINAL ACCESSION NUMBER(S) C
Page 45 and 46: 23. Donovan, W. P., J. M. González
Page 47 and 48: 66. Masson, L., A. Mazza, L. Gringo
Page 49 and 50: diptera-specific insecticidal endot
Page 51 and 52: NAME SOURCE STRAIN KNOWN TOXICITYCr
Page 53 and 54: Phylogram of Cry and Cyt Holotype S
Page 55 and 56: • What is the mode of action of C
Page 57 and 58: GENERAL INDEX OF STRAINS, PHAGE AND

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