pBAD/Myc - Gene Synthesis

Cloning into pBAD/His, continuedpBAD/His BMultiple Cloning Site1O 2 RegionAAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT61121181241301358412TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCAO 1 RegionAAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTGCAP binding siteATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGGI 2 and I 1 Region-35 -10ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTGGGCRBSNco IPolyhistidine RegionTAACAGGAGG AATTAACC ATG GGG GGT TCT CAT CAT CAT CAT CAT CAT GGT ATG GCTMet Gly Gly Ser His His His His His His Gly Met AlaXpress EK recognition siteEpitopeAGC ATG ACT GGT GGA CAG CAA ATG GGT CGG GAT CTG TAC GAC GAT GAC GAT AAGSer Met Thr Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp LysEK cleavage siteXho I Sac I Bgl II Pst I Pvu II Kpn IEcoR ISfu I Hind IIIGAT CCG AGC TCG AGA TCT GCA GCT GGT ACC ATA TGG GAA TTC GAA GCT TGGAsp Pro Ser Ser Arg Ser Ala Ala Gly Thr Ile Trp Glu Phe Glu Ala Trp463CTGTTTTG GCGGATGAGA GAAGATTTTC AGCCTGATAC AGATTAAATC AGAACGCAGA521AGCGGTCTGA TAAAACAGAA TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC ACCTGACCCC581rrnB T1 and T2 transcriptional terminatorATGCCGAACT CAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC TCCCCATGCG641AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT701TCGTTTTATC TGTTGTTTG10