pBAD/Myc - Gene Synthesis
pBAD/Myc - Gene Synthesis
pBAD/Myc - Gene Synthesis
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Cloning into <strong>pBAD</strong>/<strong>Myc</strong>-His, continued<strong>pBAD</strong>/<strong>Myc</strong>-His CMultiple Cloning Site1O 2 RegionAAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT61121181241301362415472TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCAO1 RegionAAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTGCAP binding siteATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGGI1 and I2 Region-35 -10ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTGGGCRBSNco I Xho I Sac I Bgl II Pst I Kpn I EcoR ITAACAGGAGG AATTAACC ATG GATCCGAGCT CGAGATCTGC AGCTGGTACC ATATGGGAATMetSfu I Hind III SnaB Imyc epitopeTCGAAGCTTA CGTA GAA CAA AAA CTC ATC TCA GAA GAG GAT CTG AAT AGC GCCGlu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Ser AlaPolyhistidine RegionGTC GAC CAT CAT CAT CAT CAT CAT TGA GTTTAAACGG TCTCCAGCTT GGCTGTTTTGVal Asp His His His His His His ***GCGGATGAGA GAAGATTTTC AGCCTGATAC AGATTAAATC AGAACGCAGA AGCGGTCTGA532TAAAACAGAA TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACT592rrnB T 1 and T2 transcriptional terminatorsCAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC TCCCCATGCG AGAGTAGGGA652ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC15
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