pBAD/Myc - Gene Synthesis
pBAD/Myc - Gene Synthesis
pBAD/Myc - Gene Synthesis
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Appendix<strong>pBAD</strong>/His/lacZDescription<strong>pBAD</strong>/His/lacZ is a 7115 bp control vector containing the gene for β-galactosidase fusedto the N-terminal peptide. It was constructed by digesting the vector pTrcHis/lacZ withNco I and Nsi I to remove the lacI q gene and the trc promoter and replacing with anNco I-Nsi I fragment containing the araC gene and the araBAD promoter. The β-galactosidaseportion of the fusion may be released by digestion with BamH I and Hind III. Thevector expresses a 120 kDa protein.Map ofControl VectorThe figure below summarizes the features of the <strong>pBAD</strong>/His/lacZ vector. The completenucleotide sequence for <strong>pBAD</strong>/His/lacZ is available for downloading from ourWorld Wide Web site (www.invitrogen.com) or by contacting Technical Service (seepage 27).PBADATG6xHis Xpress EKEpitope Sitelac Z termaraC<strong>pBAD</strong>/His/lacZ7.1 kbAmpicillinComments for <strong>pBAD</strong>/His/lacZ7115 nucleotidespBR322 oriaraBAD promoter region: bases 4-276Initiation ATG: bases 320-322Polyhistidine tag: bases 332-349Xpress epitope: bases 389-412Enterokinase recognition site: bases 398-412LacZ ORF: bases 419-3475rrnB transcription termination region: bases 3565-3722Ampicillin ORF: bases 4002-4862pBR322 origin: bases 5007-5680AraC ORF: bases 7089-621122
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