pBAD/Myc - Gene Synthesis

Cloning into pBAD/Myc-His, continuedpBAD/Myc-His BMultiple Cloning Site1O2 RegionAAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT61121181241TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCAO1 RegionAAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTGCAP binding siteATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGGI2 and I1 Region-35 -10ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTGGGC301357414RBSNco I Xho I Sac I Bgl II Pst I Kpn ITAACAGGAGG AATTAACC ATG GATCCGAGCT CGAGATCTGC AGCTGGTACC ATATGMetEcoR I Sfu I Hind III Xba Imyc epitopeGGAATTCGAA GCTTTCTA GAA CAA AAA CTC ATC TCA GAA GAG GAT CTG AAT AGC GCCGlu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Ser AlaPolyhistidine RegionGTC GAC CAT CAT CAT CAT CAT CAT TGA GTTTAAACGG TCTCCAGCTT GGCTGTTTTGVal Asp His His His His His His ***471GCGGATGAGA GAAGATTTTC AGCCTGATAC AGATTAAATC AGAACGCAGA AGCGGTCTGA531591TAAAACAGAA TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACTrrnB T1 and T2 transcriptional terminatorsCAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC TCCCCATGCG AGAGTAGGGA651ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC14