pBAD/Myc - Gene Synthesis
pBAD/Myc - Gene Synthesis
pBAD/Myc - Gene Synthesis
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Table of ContentsTable of Contents................................................................................................................................................iiiImportant Information......................................................................................................................................... ivIntroduction.................................................................................................................. 1Overview.............................................................................................................................................................. 1<strong>pBAD</strong>/His Vector ................................................................................................................................................ 3<strong>pBAD</strong>/<strong>Myc</strong>-His Vector ........................................................................................................................................ 5Methods........................................................................................................................ 7<strong>Gene</strong>ral Cloning................................................................................................................................................... 7Cloning into <strong>pBAD</strong>/His ....................................................................................................................................... 8Cloning into <strong>pBAD</strong>/<strong>Myc</strong>-His ............................................................................................................................. 12E. coli Transformation ....................................................................................................................................... 16Expression.......................................................................................................................................................... 17Purification......................................................................................................................................................... 21Appendix .................................................................................................................... 22<strong>pBAD</strong>/His/lacZ.................................................................................................................................................. 22<strong>pBAD</strong>/<strong>Myc</strong>-His/lacZ.......................................................................................................................................... 23Recipes............................................................................................................................................................... 24Technical Service............................................................................................................................................... 27References.......................................................................................................................................................... 30iii
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