pBAD/Myc - Gene Synthesis
pBAD/Myc - Gene Synthesis
pBAD/Myc - Gene Synthesis
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<strong>pBAD</strong>/<strong>Myc</strong>-His VectorFeatures of<strong>pBAD</strong>/<strong>Myc</strong>-HisThe important elements of <strong>pBAD</strong>/<strong>Myc</strong>-His A (4094 bp), <strong>pBAD</strong>/<strong>Myc</strong>-His B (4092 bp),and <strong>pBAD</strong>/<strong>Myc</strong>-His C (4093 bp) are described in the following table. All features havebeen functionally tested.FeaturearaBAD promoter (P BAD)Optimized ribosome binding siteInitiation ATGMultiple cloning siteC-terminal myc epitope tag(Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu)C-terminal polyhistidine regionrrnB transcription termination regionBenefitProvides tight, dose-dependent regulation ofheterologous gene expression (Guzman etal., 1995)Increases efficiency of recombinant fusionprotein expressionProvides a translational initiation site for thefusion proteinAllows insertion of your gene for expressionAllows detection of the fusion protein by theAnti-<strong>Myc</strong> Antibody (Catalog no. R950-25)(Evans et al., 1985)Forms metal-binding site for affinitypurification of recombinant fusion proteinon metal-chelating resin (i.e. ProBond )In addition, it allows detection of therecombinant protein with Anti-His (C-term)Antibody (see page 2)Strong transcription termination regionAmpicillin resistance gene (β-lactamase) Allows selection of the plasmid in E. colipBR322 originaraC geneLow copy replication and growth in E. coliEncodes the regulatory protein for tightregulation of the P BAD promoter (Lee, 1980;Schleif, 1992)continued on next page5
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