pBAD/Myc - Gene Synthesis
pBAD/Myc - Gene Synthesis
pBAD/Myc - Gene Synthesis
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<strong>pBAD</strong>/<strong>Myc</strong>-His/lacZDescription<strong>pBAD</strong>/<strong>Myc</strong>-His/lacZ is a 7242 bp control vector containing the gene for β-galactosidasefused to the C-terminal peptide. It was constructed by digesting the vector pTrcHis2/lacZwith Nco I and Nsi I to remove the lacI q gene and the trc promoter and replacing with anNco I-Nsi I fragment containing the araC gene and the araBAD promoter. The β-galactosidaseportion of the fusion may be released by digestion with Sfu I (BstB I). Other cloningoptions are possible. The vector expresses a 120 kDa protein.Map ofControl VectorThe figure below summarizes the features of the <strong>pBAD</strong>/<strong>Myc</strong>-His/lacZ vector. Thecomplete nucleotide sequence for <strong>pBAD</strong>/<strong>Myc</strong>-His/lacZ is available for downloadingfrom our World Wide Web site (www.invitrogen.com) or by contacting TechnicalService (see page 27).Nco ISfu Ilac ZPst IXho IBgl IIPst ISfu IHind IIIPBADATG myc 6xHistermaraC<strong>pBAD</strong>/<strong>Myc</strong>-His/lacZ7.2 kbAmpicillinComments for <strong>pBAD</strong>/<strong>Myc</strong>-His/lacZ7241 nucleotidespBR322 oriaraBAD promoter region: bases 4-276Initiation ATG: bases 319-321LacZ ORF: bases 373-3429myc epitope: bases 3523-3552Polyhistidine tag: bases 3568-3585rrnB transcription termination region: bases 3691-3848Ampicillin ORF: bases 4128-4988pBR322 origin: bases 5133-5806AraC ORF: bases 7215-633723
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