Report on Harmonisation of freshwater biological methods

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49. The Danish Stream Fauna Index is determined on the basis of indicator taxa andthe number of diversity groups in the total fauna sample (kick samples and handpickedsamples). The first step of assessing the quality class is done by assigning thesampled fauna to one of six indicator groups based on at least two specimens ofselected taxonomic groups found in the kick samples or one specimen found in thehand-picked sample, respectively. Additionally, selected species belong to positive(e.g. Baetidae, Nemoura) or negative (e.g. Erpobdella, Sialis) diversity groups, whichare added up and classified (four ranges in total) within the second step of assessment.Carrying-out both steps leads directly to one of seven quality classes, ranging from'unimpacted' to 'very strongly impacted'.Estonia50. The Estonian quality assessment of watercourses is based on the Swedish BenthicFauna in Lake Littorals and Running Water - Time Series method. It uses fourselected indicator metrics of macroinvertebrate diversity and distribution: Shannon'sDiversity Index, ASPT Index, Danish Stream Fauna Index and Acidity Index. Thismultimetric approach leads to an assessment of both watercourses (riffle areas) andlittoral zone of lakes, resulting in one of five quality classes. It is planned to use theratio between observed index value and expected reference value to indicate theextent to which bottom fauna conditions deviate from an undisturbed (natural) state.Finland51. Assessment is based on riffle/ rapid kick net samples with three replicates (Finishsatandard SFS – 5077). A multimetric method for assessing the status ofmacroinvertebrates has been tested. The system under preparation consists of severalmetrics, e.g. number of type-specific species, EPT taxons and abundance of indicatorspecies.France52. The Standardised Biological Global Index (I.B.G.N.) is widely used in monitoringprogrammes in France since 1992. Benthic invertebrates are usually identified tofamily-level, some groups to higher taxonomical level. The method embraces 138different taxa to determine the ‘total variety of the sample (t)’ split up into 14 varietyclasses. 38 taxa constitute nine ‘faunal indicator groups (GI)’, which are selected if78
three (or ten, respectively) individuals belonging to an indicator taxon are found in thesample. On the basis of an index value-chart the I.B.G.N. is calculated directly -dependent on variety class (table rows) and indicator group (table columns) andassigned to 5 quality classes.53. The Oligochaeta Index for Sediment Bioindication (I.O.B.S.) assesses the generalquality of stable and permanent fine sediments of natural and artificial watercoursesand indicates their susceptibility to gross organic stress and micropollutants such asmetals and PCBs. To calculate the index 100 individuals have to be identified. Thetotal number of taxa (only Oligochaeta) and the dominant percentage of Tubificidaewith or without hair-chaetae are determined. The latter group indicates the effect ofmicropollutants.Germany54. For WFD implementation a multimetric approach for biological classificationbased on benthic invertebrates was developed for selected stream types across Europe(AQEM-Project, Hering et al., 2004). In Germany, this approach is now beingexpanded to cover all stream types that have been identified for WFDimplementation. The method includes modules for the detection of organic pollution,acidification and general degradation. The method will undergo testing regarding itspracticability in 2005 and 2006.55. Part of the multimetric scheme is the Potamon-Characterisation-Index, which isspecifically designed for the ecological status assessment of large rivers and is basedon the presence of typical species of the potamon (lower course of the river).Greece56. In Greece, no generally applied assessment method exists. The high proportion ofendemic species necessitates thorough autecological studies prior to the developmentof evaluation methodologies. Additionally, a sampling network has to be established.57. The European AQEM project generated a multimetric classification scheme forthree Greek stream types based on macroinvertebrate sampling. Its application isunder development. The Hellenic Evaluation System is an adaptation of the BMWP-ASPT score to Greece conditions.79
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Institute for Environment and Susta
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CONTENTSBackground and purpose of t
Page 5 and 6:
Background and purpose of the docum
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States and candidate countries. Inf
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classification, each of these being
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BIOLOGICAL QUALITY ELEMENT: PHYTOPL
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indicators, species lists, frequenc
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53. The identification and enumerat
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64. In general, this technique is t
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RIVER BIOLOGICAL QUALITY ELEMENT: F
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84. The development of specific sta
Page 23 and 24:
practice guides for identification
Page 25 and 26:
100. The information received from
Page 27 and 28: Evaluation of the suitability of cu
Page 29 and 30: group should update its primary fin
Page 31 and 32: ReferencesAFNOR (Association Franç
Page 33 and 34: EN ISO 8689-2 Water quality - Biolo
Page 35 and 36: Lazaridou-Dimitriadou, M., C. Kouko
Page 37 and 38: Shannon, C.E. and W. Weaver, 1949.
Page 39: Annex I: Composition of the Geograp
Page 43 and 44: Annex III: River biological assessm
Page 45 and 46: Annex IV: Analysis of lake biologic
Page 47 and 48: Number of sampling stations10080%60
Page 49 and 50: IT 90% acetone spectrophotometricPT
Page 51 and 52: 10. The sampling depth and volume s
Page 53 and 54: PTESFIIE5667-2/98 Romanianstandardi
Page 55 and 56: Sampling stations%1008060402001 2-1
Page 57 and 58: MACROPHYTES16. The aquatic Macrophy
Page 59 and 60: Plants sampled per GIG1008060%40Eme
Page 61 and 62: NO qualitativ method species number
Page 63 and 64: indicators, species lists, frequenc
Page 65 and 66: 26. The sampling frequency is varia
Page 67: CEN/TC 230/WG 2/ TG 4 N28, 2 nd wor
Page 70 and 71: programs are based only on the diat
Page 72 and 73: 21. Some countries like France, Est
Page 74 and 75: Table 1. European methods for monit
Page 76 and 77: countries also covers Non-EU Member
Page 80 and 81: Hungary58. Since 2002 a modificatio
Page 82 and 83: Acidification Index, based on the s
Page 84 and 85: Identification is predominantly to
Page 86 and 87: size of the net range between 250 t
Page 88 and 89: water bug genus (Aphelocheirus) and
Page 90 and 91: Table 5. Common abundance classific
Page 92 and 93: 108. Process Assessment focuses on
Page 94 and 95: Austria120. MuLFA: Ecological Integ
Page 96 and 97: Sweden126. Swedish fish Index: Appe
Page 98 and 99: ut comparisons have been made with
Page 100 and 101: seasons for sampling are summer and
Page 102 and 103: 102
Page 104 and 105: Consultation open to ECOSTAT &inter
Page 106 and 107: Table 1. List of European standards
Page 108 and 109: 108
Page 110 and 111: development of typologySweden Yes,
Page 112 and 113: one or other option depends on the
Page 114 and 115: 114
Page 116 and 117: • An integrated holistic evaluati
Page 118 and 119: • Phytoplankton: Yes; Clorophyll
Page 120 and 121: • Macroalgae: No• Benthic inver
Page 122 and 123: • Macrophytes: No• Macroalgae:
Page 124: Mission of the JRCThe mission of th
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