Post-transcriptional control of gene expression

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miRNA: definition and processing• miRNA are short endogenous noncoding RNA molecules. Function asguide molecules in diverse silencing pathways• miRNAs genes are transcribed from DNA, but are not translated• The DNA sequence that codes for an miRNA gene is longer than themiRNA. This forms a primary miRNA structure (pri-miRNA) which is adouble stranded RNA hairpin loop• In ANIMALS, the nuclear enzyme Drosha cleaves the base of the hairpin toform pre-miRNA. The pre-miRNA molecule is then actively transported outof the nucleus into the cytoplasm by Exportin 5, a carrier protein.• The Dicer enzyme cuts 20-25 nucleotides from the base of the hairpin torelease the mature miRNA.• In PLANTS, which lack Drosha homologues, pri- and pre-miRNAprocessing by Dicer probably, takes place in the nucleus, and maturemiRNA duplexes are exported to the cytosol by Exportin 5.
Examples of Metazoan pri-miRNAsLin-4 RNALin-7 RNAThe initial stem−loop configurationof the primary transcript providesstructural clues that have been usedto guide searches of genomic sequencefor candidate miRNA genes
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Post-transcriptional control of gene expression

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