Annex White book.pdf

A4.1.2.5 Foreign materialsThe term “foreign materials” refers to the leaves, stems, straws andany other vegetable material. The only permissible mineral materialis sand, soil and dust. They should be removed according to the ISO927:1982 procedure.3 g of a saffron sample are weighed on a precision scale (variation ±0,01 g). The sample is then spread out on a grey paper. With a pair ofpliers the stigmas are separated from the foreign material. The foreignmaterial is then weighed while placed on a glass plate that has beenalready weighed. The amount of foreign material is calculated accordingto following formula: WME = (m2 – m1) x (100/m0)%. m0 is the massof the saffron sample rendered in gram, m1 is the mass of the glassplate in gram and m2 is the mass of the glass plate together with theforeign material. The outcome is rendered in double decimal percentage(m/m).A4.1.2.6 Extract soluble in cold water, in dry matterThe maximum content of extract soluble in cold water is determinedin a 2 gram sample according to ISO 941:1980. The extract lies at approximately65%.The applied procedure is as follows: a sample of 2 g is weighed witha variation of 1 mg. The sample is placed in a volumetric 100 ml bottle.The bottle is filled with distilled water according to ISO 3696:1996and sealed. The bottle is agitated for 1 minute every 30 minutes for theduration of 8 hours. The solution rests for 16 hours with no furtheragitation. The extract is filtered through an average porosity paper filter.An infiltrate fraction of 50 ml is received which is placed in capsule thathas been already dried and weighted accurately (deviation 1 mg). Theinfiltrate is evaporated and dried in a boiling bath. The capsule with theextract is heated in a furnace for 1 hour at 103oC ±2oC. The sample isthen cooled in the dryer and weighed. Heating, cooling and weighingare repeated until the difference between two continuous weightings isless than 2 mg.The extract soluble in cold water and rendered in mass percentageof the dry matter is calculated according to following formula: WES =(PF – Po/ PM) x (100/100-H) x 200%. PF is the capsule weight togetherwith the extract, Po is the weight of the sole capsule, PM is the sampleweight and H is the humidity content of the sample. The outcome isrendered in double decimal percentage (m/m).150