Annex White book.pdf

- Injection volume: 50 ml- Moving phase flow: 0,7 ml/minuteSolvents:- Phase A: acetified water HPLC (165 ml of acetic acid in 1000 mlof water)- Phase B: acetonitrile (HPLC quality)Prior the chromatographic analysis, the column is prepared with 5ml of acetonitrile / water (50 / 50) and 5 ml of water of HPLC quality,with 0,5 ml/minute flow.Phase Duration (min) Phase A Phase BEquilibrium 30 701 0 30 702 30 5 953 40 0 1004 52 0 100Table 12. SeparationdevelopmentThe possible artificial dye substances contained in the sampleare identified by comparing the absorption time and the UV-Visspectrums between 300 and 700 nm with those of the dissolutionpattern.166