The partial toxicity tests of copper (Cu 2+ ), zinc (Zn 2+ ) and cyanide (CN - ) for young cobia(<strong>Rachycentron</strong> <strong>canadum</strong>) fishes 45 days-old were conducted in Doson station during ten days (yr2005). These three toxic substances affected strongly the growth rate of the Cobia fish (length andweight of the body), even at the lowest treated concentration. The LC values of copper, zinc andcyanide were 0.32 mg l -1 ; over mg l -1 and 0.049 mg l -1 , respectively. Based on the statistical analysisof growth rate of cobia by determining IC25, IC50, NOEC and LOEC values, we have addressedthe safe concentrations of these two heavy metals and cyanide in the environmental aquaculturewhich must be lower than 0.025 mg Cu l -1 , 0.5 mg Zn l -1 and 0.005 mg CN l -1 , respectively. Theresult also showed that cyanide was the most toxic to Cobia, followed by copper and zinc.24. Fang, F., Xu, M., Cai, Q. & Huang, X. (2007). Analysis and comparison on level of 5 kinds ofhormones in muscles of cultured and wild fishes in Zhanjiang sea area. Journal of TropicalOceanography, 26(5), 69-72.The levels of growth hormone, estradiol, progesterone, testosterone and chorlonic gonadotropichormone in the muscles of cultured and wild Sparus macroccephalus, Plectorhynchus cinctus,<strong>Rachycentron</strong> <strong>canadum</strong>, Nibea japonica, Pampus argenteus in the Zhanjiang sea area weredetermined by radioimmunoassay (RIA). The result showed that the level of each hormone wasdifferent with different fish species, and their distributions were as follows. The highest levels ofgrowth hormone, estradiol, progesterone, testosterone and chorionic gonadotropic hormone in the 5species of cultured and wild fishes occurred in cultured and wild Nibea japonica, cultured Sparusmacroccephalus and wild <strong>Rachycentron</strong> <strong>canadum</strong>, cultured and wild <strong>Rachycentron</strong> <strong>canadum</strong>,cultured and wild Pampus argenteus, cultured Nibea japonica and wild Plectorhynchus cinctusrespectively. The level of each hormone in the cultured fish was obviously higher than the samespecies of the wild fish (p
26. Feng, Y., Su, Y., Guo, Z., Xu, L., Sun, X. & Wang, Y. (2013). Identification and expressionanalysis of a CC chemokine from cobia (<strong>Rachycentron</strong> <strong>canadum</strong>). Fish Physiology andBiochemistry, 39(3), 459-469.Chemokines are small, secreted cytokine peptides known principally for their ability to inducemigration and activation of leukocyte populations and regulate the immune response mechanisms.The cobia (<strong>Rachycentron</strong> <strong>canadum</strong>), a marine finfish species, has a great potential for net cageaquaculture in the South China Sea. We isolated and characterized a CC chemokine cDNA fromcobia-designated RcCC2. Its cDNA is 783 bp in length and encodes a putative protein of 110 aminoacids. Homology and phylogenetic analysis revealed that the RcCC2 gene, which contains fourconserved cysteine residues, shares a high degree of similarity with other known CC chemokinesequences and is closest to the CCL19/21 clade. The mRNA of RcCC2 is expressed constitutivelyin all tested tissues, including gill, liver, muscle, spleen, kidney, head kidney, skin, brain, stomach,intestine and heart, but not blood, with the highest level of expression in gill and liver. The reversetranscription quantitative polymerase chain reaction was used to examine the expression of theRcCC2 gene in immune-related tissues, including head kidney, spleen and liver, followingintraperitoneal injection of the viral mimic polyriboinosinic polyribocytidylic acid, formalin-killedVibrio carchariae (bacterial vaccine) and phosphate-buffered saline as a control. RcCC2 geneexpression was up-regulated differentially in head kidney, spleen and liver during 12 h afterchallenge. These results indicate that the RcCC2 gene is inducible and is involved in immuneresponses, suggesting RcCC2 has an important role in the early stage of viral and bacterialinfections.27. Franks, J.S. (1995). A pugheaded cobia (<strong>Rachycentron</strong> <strong>canadum</strong>) from the north central Gulf ofMexico. Gulf Research Reports, 9(2), 143-145.A pugheaded cobia (<strong>Rachycentron</strong> <strong>canadum</strong>) captured in the Northcentral Gulf of Mexicorepresents the first record of pugheaded in cobia. The specimen, a 4-year-old gravid female,exhibited considerable distortion of the premaxillary and maxillary bones, with the length of thesnout 46% shorter than that of a normal cobia of the same length. The anomaly had no apparenteffect on feeding, since the stomach contained a substantial amount of food, and the fish was thesame length expected of a normal 4-year-old cobia.28. Franks, J.S. & Brown-Peterson, N.J. (2002). A review of age, growth, and reproduction of Cobia,<strong>Rachycentron</strong> <strong>canadum</strong>, from U.S. waters of the Gulf of Mexico and Atlantic Ocean. In:Proceedings of the Gulf and Caribbean Fisheries Institute,53, 553-569.A review of available scientific information on the age, growth, and reproduction of cobia,<strong>Rachycentron</strong> <strong>canadum</strong>, from United States waters of the Gulf of Mexico and Atlantic Ocean isprovided. Periodicity of annulus formation on sectioned sagittae has been partially validated bymarginal-increment analysis, and age in years is estimated as the number of observed opaque bands.Growth in length for both sexes is rapid through age 2. Females grow faster and live longer thanmales and dominate all age groups. Gulf males reached age 9 and 1,390 mm FL; females reachedage 11 and 1,651 mm FL. Atlantic males reached age 14 and 1,360 mm FL; females reached age 13and 1,420 mm FL. Ages 2 - 5 dominated the age structure of Gulf and Atlantic samples. Cobia werefully recruited to the northeastern Gulf recreational fishery at age 4, and the instantaneous rate oftotal mortality (Z) estimated for fully recruited cobia ages 4 - 8 was 0.75. Cobia have an extendedspawning season throughout their range in United States waters, averaging five months (mid-April-August) in the Atlantic Ocean and six months (April-September) in the Gulf of Mexico. Some Gulffemales appear to cease spawning by July. Female cobia can obtain sexual maturity as small as 700mm FL. Histological analysis shows cobia are a multiple spawning species; females are estimatedto spawn once every five days throughout most of the U.S. region. Batch fecundity increasessignificantly with FL and ovary-free body weight (OFBW); mean relative batch fecundity is 53.1 ±9.4 eggs/g OFBW. While the testis of males contain sperm year-round, spermatogenesis only takesplace from February - August, and spermatogonial proliferation is observed during non-spawningmonths.29. Franks, J.S., Garber, N.M., & Warren, J.R. (1996). Stomach contents of juvenile cobia,<strong>Rachycentron</strong> <strong>canadum</strong>, from the northern Gulf of Mexico. Fishery Bulletin, 94(2), 374-380.This paper represents the first study describing specifically the diet of juvenile <strong>Rachycentron</strong><strong>canadum</strong>.8
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190. Zhang, H., Mao, L., Feng, J.,
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This suggests that the enrichment o
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formerly characterized elovl5 elong
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208. Weirich, C.R., Stokes, A.D., S
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trypsin activities of intestine of
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decreased gradually as fish body we
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BIA methodology can be utilized as
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cobia were 21.72 mg kg -1 , 22.38 m
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effects upon final product quality,
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than the optimal requirement of cob
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A 9-week feeding trial was conducte
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soybean meal in Cobia, Rachycentron
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261. Sun, L., Chen, H., Huang, L.,
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fishes and invertebrates. Here we i
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274. Watson, A.M., Buentello, A. &
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estimated to be 44.7 mg kg -1 based
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20% of alternative protein meal, ne
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levels of methionine (0.61%, 0.83%,
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298. Mach, D.T.N. & Nortvedt, R. (2
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acids (FFA), peroxide value (PV), t
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068Breitenbach, B.078Brenkert, K.01
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271Duncan, M.226Dung, L.Q.023DuPaul
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139, 149Kilduff, P.180Kim, I.H.088K
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265Myrseth, B.140Nabavi, S.M.B.001,
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244Shi, C.071Shi, G.218, 262, 287Sh
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291Xie, J.269, 270Xu, H.037, 190Xu,