of IMP to inosine and hypoxanthine took place, after decomposition, and hypoxanthine became thedominant compound. The K value of 27% was proposed as the limit of quality acceptability.Conclusively, due to its good taste and high lipid content, this fish is suitable for serving as sashimi(raw fish), roasted, and smoked products.57. Su, Y., Feng, J., Guo, Z., Xi, L. & Wang, J. (2008). Morphological studies on the development oflymphoid organs in cobia <strong>Rachycentron</strong> <strong>canadum</strong>. Marine Fisheries Research, 29(4), 7-14.Histological and histochemical methods were applied to study the development of the head kidney,spleen and thymus in cobia <strong>Rachycentron</strong> <strong>canadum</strong>, during 1-44 day posthatch (dph). Thepronephric ducts were first observed, near which some primordial haemopoietic stem cells wereobserved 3 d later. The haemopoietic stem cells gradually differentiated into erythrocytes and largelymphocytes. The spleen developed later, and soon became rich in blood capillaries anderythrocytes, but had only a few macrophage. The thymus was the last lymphoid organ appearedbut developed very quickly. An outer zone and inner zone in the thymus could be observed clearly.It was concluded that the sequence of appearance of lymphoid organs, from first to last, was headkidney, spleen and thymus. Before the small lymphocytes developed, cellular types involved innon-specific immunity mechanisms, such as macrophage and reticular cells, were observed in thelymphoid organs. The respective lymphoid organ anlage became thymus, head kidney and spleensuccessively. No plasma cells were observed in the samples, indicating that the lymphoid organswere not fully grown during this period.58. Su, Y., Guo, Z., Xu, L., Jiang, J., Wang, J. & Feng, Y. (2012). Identification of a cobia(<strong>Rachycentron</strong> <strong>canadum</strong>) CC chemokine gene and its involvement in the inflammatory response.Fish & Shellfish Immunology, 32(1): 204-210.The chemokines regulate immune cell migration under inflammatory and physiological conditions.We investigated a CC chemokine gene (RcCC1) from cobia (<strong>Rachycentron</strong> <strong>canadum</strong>). The fulllengthRcCC1 cDNA is comprised 673 nucleotides and encodes a four-cysteine arrangement 99-amino-acid protein typical of known CC chemokines. The genomic DNA of RcCC1 consists ofthree exons and two introns. Phylogenetic analysis showed that RcCC1 was closest to the MIPgroup of CC chemokines. Quantitative real-time RT-PCR (qRT-PCR) analysis revealed RcCC1 wasconstitutively expressed in all tissues examined, with relative strong expression in gill, blood,kidney, spleen, and head kidney. The RcCC1 transcripts in the head kidney, spleen, and liver werequickly up-regulated after stimulation with formalin-inactivated Vibrio carchariae (bacterialvaccine) or polyriboinosinic polyribocytidylic acid (poly I:C). These results indicate RcCC1 notonly plays a role in homeostasis, but also may be involved in inflammatory responses to bacterialand viral infection.59. Su, Y., Xu, L., Feng, J., Guo, Z. & Wang, J. (2007). Study on the morphology of peripheral bloodcells of juvenile cobia <strong>Rachycentron</strong> <strong>canadum</strong>. South China Fisheries Science, 3(1), 48-53.The peripheral blood was collected from cobia (<strong>Rachycentron</strong> <strong>canadum</strong>) on the 60th day afterhatching, and blood cells were studied by light microscope. Five types of blood cells were observedas follow: erythrocyte, lymphocyte, monocyte, neutrophil and thrombus cells. The erythrocytecounts and leukocyte counts of cobia was 2.97 ± 0.82 x 10 9 ind. mL -1 and 1.39 ± 0.94 x 10 6 ind. mL -1 , respectively. The percentages of lymphocytes, neutrophils, monocytes and thrombocytes in totalleucocytes, were (53.00 ± 8.51)%, (17.59 ± 4.28)%, (4.78 ± 1.37)% and (24.63 ± 4.08)%,respectively. Neither eosinophil nor basophil was found. The quantity of erythrocytes was muchlarger than that of leucocytes. Among the leucocytes, the lymphocytes were the most abundant,while that of monocytes was the least. The immatured and dividing erythrocyte were found. Thesmall lymphocytes with pseudopod and large lymphocytes with the villus were observed also. Inaddition, the monocyte, neutrophil and thrombu cells were recognized by different shapes ofnucleolus.60. Sun, L., Chen, H. & Huang, L. (2006). Effect of temperature on growth and energy budget ofjuvenile cobia (<strong>Rachycentron</strong> <strong>canadum</strong>). Aquaculture 261(3), 872-878.Growth and energy budget of juvenile cobia (initial body weight ~22 g) at various temperatures (23,27, 31 and 35 °C) were investigated in this study. Maximal ration level (RL max , %/day) increased astemperature (T, °C) increased from 23 °C to 31 °C but decreased at 35 °C, described as a quadraticequation: RL max = -0.023T 2 + 1.495T – 17.52. Faecal production (f, mg g -1 day -1 ) increased withincreased temperature (T, °C), described as a power function: lnf = 0.738lnT – 0.806. Astemperature increased, feed absorption efficiency in dry weight (FAE d , %), protein (FAE p , %) and17
energy (FAE e , %) all increased first and then decreased, but the variation of feed absorptionefficiency was small, with ranges of 89.59-91.08%, 92.91-94.71%, 93.92-95.32%, respectively.Specific growth rate in wet weight (SGR w , %/day), dry weight (SGR d , %/day), protein (SGR p ,%/day) and energy (SGR e , %/day) showed a domed curve relative to temperature (T, °C), describedas quadratic equations: SGR sub w = -0.068T 2 + 3.878T – 50.53, SGR d = -0.079T 2 + 4.536T – 59.64,SGR p = -0.084T 2 + 4.783T – 63.08 and SGR e = -0.082T 2 + 4.654T – 60.99, and SGR w , SGR d , SGR pand SGR e maximized at 28.5 °C, 28.6 °C, 28.4 °C, 28.5 °C, respectively, as calculated from theregression equations. The relationships between feed conversion efficiency in wet weight (FCE w ,%), dry weight (FCE d , %), protein (FCE p , %), energy (FCE e , %) and temperature (T, °C) also tookon a domed curve described as quadratic equations: FCE w = -0.726T 2 + 39.71T – 473.8, FCE d = -0.276T 2 + 15.31T – 190.6, FCE p = -0.397T 2 + 22.05T – 277.9 and FCEe = -0.350T 2 + 19.39T –239.9, and FCE w , FCE d , FCE p and FCE e maximized at 27.4 °C, 27.8 °C, 27.7 °C and 27.7 °C,respectively, as calculated from the regression equations. Energy budget of juvenile cobia fedsatiation was 100C = 5F + 67(U+R) + 28G at water temperature 27 °C and 100C = 5F + 70(U+R) +25G at water temperature 31 °C, where C is food energy, F is faeces energy, (U+R) is excretionenergy and metabolism energy, and G is growth energy.61. Trushenski, J., Schwarz, M.H., Takeuchi, R., Delbos, B. & Sampaio, L.A. (2010). Physiologicalresponses of cobia <strong>Rachycentron</strong> <strong>canadum</strong> following exposure to low water and air exposure stresschallenges. Aquaculture, 307(1-2), 173-177.Prevention is the most viable disease management strategy in aquaculture, and prevention isprimarily driven by strategies to avoid or minimize the effects of stress. Unfortunately, there is littleinformation available regarding the stress physiology of emerging aquaculture species orappropriate experimental stressing protocols for these fishes, and thus very little context in which toevaluate mitigation strategies. Accordingly, the stress response of cobia was evaluated followingexposure to 2 experimental stressors: low water and air exposure. Juveniles were exposed to air for1min (AIR EXPOSURE), held for 15min in water lowered to the fish's lateral midline (LOWWATER), or unchallenged (CONTROL) prior to the collection of blood samples at 0 (prechallenge),0.5, 1, 2, 6, 12, 24, 48, and 72h post-challenge. Both stressors elicited classicalhaematological changes indicative of the generalized stress response, however, the magnitude of theresponse was consistently greater in the AIR EXPOSURE group. Plasma cortisol, glucose, andlactate concentrations increased rapidly in the AIR EXPOSURE and LOW WATER groups,peaking within 1h of challenge. Cortisol returned to basal levels rapidly, whereas glucose andlactate remained elevated for a longer period of time. Regardless of the stressor used, fish recoveredwithin 12h of the challenge. The primary and secondary responses of juvenile cobia challenged withlow water and air exposure appear to respond in a similar fashion to other species exposed to theseexperimental stressors. Both low water and air exposure are suitable experimental stressors for usein cobia based on their ability to induce a classical stress response and ease of implementation.62. Valinassab, T., Ashtari, S., Sedghi, N. & Daghoghi, B. (2008). Reproductive biology of<strong>Rachycentron</strong> <strong>canadum</strong> in the Persian Gulf (Hormozgan Province waters). Iranian ScientificFisheries Journal, 17(2), 143-152.We collected 478 specimens of Cobia, <strong>Rachycentron</strong> <strong>canadum</strong>, from Hormozgan province watersduring October 2005~September 2006, and studied reproductive parameters such as spawningseason, sex ratio, maturity stages, fecundity and ova diameter. The maturity and spawning seasonwere investigated through macroscopic and microscopic (ovarians histology) observation. Studyingaverage Gonado-Somatic Index (GSI), the percent of maturity stages and ova diameter averagechanges revealed that the spawning occurred from July to the beginning of September. The totalmale to female sex ratio was 1.3:1.0 which was significantly different from the normal ratio, 1:1(P
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decreased gradually as fish body we
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BIA methodology can be utilized as
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cobia were 21.72 mg kg -1 , 22.38 m
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effects upon final product quality,
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than the optimal requirement of cob
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A 9-week feeding trial was conducte
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soybean meal in Cobia, Rachycentron
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261. Sun, L., Chen, H., Huang, L.,
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fishes and invertebrates. Here we i
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274. Watson, A.M., Buentello, A. &
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estimated to be 44.7 mg kg -1 based
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20% of alternative protein meal, ne
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levels of methionine (0.61%, 0.83%,
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298. Mach, D.T.N. & Nortvedt, R. (2
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acids (FFA), peroxide value (PV), t
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068Breitenbach, B.078Brenkert, K.01
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271Duncan, M.226Dung, L.Q.023DuPaul
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139, 149Kilduff, P.180Kim, I.H.088K
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265Myrseth, B.140Nabavi, S.M.B.001,
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244Shi, C.071Shi, G.218, 262, 287Sh
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291Xie, J.269, 270Xu, H.037, 190Xu,