within 72 hours. This phospholipase may play an important role in the pathogenesis of P. damselaesubsp. piscicida infection in the cobia.97. Liu, P.C., Lin, J., Chuang, W. & Lee, K.K. (2004). Isolation and characterization of pathogenicVibrio harveyi (V. carchariae) from the farmed marine cobia fish <strong>Rachycentron</strong> <strong>canadum</strong> L. withgastroenteritis syndrome. World Journal of Microbiology & Biotechnology, 20(5), 495-499.An outbreak of serious mortality among the cultivated juvenile cobia <strong>Rachycentron</strong> <strong>canadum</strong> L.(weighing 8-10 g) characterized by a swollen intestine containing transparent yellow fluid (ascitesand gastroenteritis) occurred in August 2001 in Taiwan. Ten motile bacterial strains, C3d1-C3d10,were isolated from head kidney (an organ located near the head of the fish) and/or the intestinalyellow fluid on tryptic soy agar supplemented with 1% NaCl (TSA1) and/or thiosulphate citrate bilesalt sucrose (TCBS) agar plates. These strains were characterized and identified as Vibrio harveyi(V. carchariae) on the basis of biochemical characteristics, and comparisons with those of threereference strains, originally identified as V. harveyi or V. carchariae. The strain C3d1 was selectedas a representative strain for virulence tests and was found lethal to the cobia with an LD 50 value of7.48×10 4 colony forming units g -1 fish body weight. All the moribund/dead fish exhibitedgastroenteritis as that observed in natural outbreak. The same bacteria could be reisolated fromkidney and the transparent yellow fluid of swollen intestine of fish after bacterial challenge usingTSA1 and TCBS plates. This is a first report showing that V. harveyi (V. carchariae) is thecausative agent of gastroenteritis in the cobia.98. Lopez, C., Rajan, J.P.R., Lin, J.H.Y., Kuo, T.Y. & Yang, H.L. (2002). Disease outbreak inseafarmed cobia (<strong>Rachycentron</strong> <strong>canadum</strong>) associated with Vibrio spp., Photobacterium damselaessp. piscicida, mongenean and myxosporean parasites. Bulletin of the European Association of FishPathologists, 22(3): 206-211.The first documented outbreak of monogenean and myxosporean parasites associated vibriosis andphotobacteriosis in cobia (<strong>Rachycentron</strong> <strong>canadum</strong>) cultured in the Penghu islands of Taiwan isreported. Monogenean infested fish showed dark skin pigmentation, haemorrhage and severe ulceron the head. Microbial analysis of these fish revealed the presence of V. alginolyticus, V. vulnificusand V. parahaemolyticus. Internally affected fish with no apparent external indications showedpaleness of liver and kidney and white tubercles in the spleen. A pure culture of bacteria isolatedfrom these organs was biochemically charecterised as Photobacterium damselae ssp. piscicida (Ph.D. piscicida). Myxosporean parasite was found in the renal tubules.99. Lu, S.Y., Wang, Y.C., Li, J.H. Wu, C.P. & Cheng, T.C. (2005). Cloning and characterization ofMX gene from cobia. Journal of the Fisheries Society of Taiwan, 32(1), 11.The fast growth and high feed conversion rate of cobia make it become a new potential species formarine cage culture. However, the disease cause by parasites, bacteria and viruses is one of themajor hurdles of cobia culture. Because there is no cure for virus diseases, the cloning andcharacterization of MX gene that is related to virus tolerance of other land animals are conducted.The total length of MX cDNA is cloned by PCR with degenerate primers, and RACE. Six hoursafter the adding of 50ng/kl of poly I:C, the induci-bilities of MX expression are found in cobia celllines derived from brain, and kidney. Cloning and analysis of MX promoter is being conducted.100. McLean, E. & Salze, G. (2008). Parasites, diseases and deformities of cobia. Ribarstvo, 66(1), 1-16.Cobia, <strong>Rachycentron</strong> <strong>canadum</strong>, is the only member of the family Rachycentridae (OrderPerciformes) and as a warm-water fish is to be found in tropical and subtropical waters. The specieshas been reported in eastern Mediterranean waters and it is likely that in this particular case, cobiais lessespian. Cobia has been farmed in Taiwan since the early 1990s and today nascent cobiaaquaculture operations operate throughout South East and Eastern Asia, in Gulf of Mexico andCaribbean Sea as well as in the United States. Many other nations are presently consideringadopting cobia as a new species for aquaculture. Production by aquaculture experienced a 7000-foldincrease from 1995 to 2005. The increased interest in the species has evolved due in large part to itsmany excellent characteristics which include good growth, with production of 6 kg live weight fishbeing possible over a year-long production cycle. Cobia are accepting of pond, net pens andrecirculation-based culture; their fillet quality is high and meat delectable; They readily takeformulated feeds and respond well to alternate proteins in their diets. Like other species new toaquaculture however, enlarged farming activities have been accompanied by increased incidence ofcommonly-encountered and emerging diseases. As an aid to current and potential producers as well29
as researchers, the following provides an overview of the published literature on cobia diseases,parasites and physical deformities.101. Mendoza, M., Gueiza, L., Martinez, X., Caraballo, X., Rojas, J., Aranguren, L.F. & Salazar,M. (2013). A novel agent (Endozoicomonas elysicola) responsible for epitheliocystis in cobiaRachycentrum <strong>canadum</strong> larvae. Diseases of Aquatic Organisms, 106(1), 31-37.Aquaculture of cobia has gained popularity in the last decade, and this species is now farmed inseveral countries in Latin America and Asia. Despite recent improvement in production techniquesthat allowed the expansion of the industry, little is known about the diseases that affect cobia duringthe larviculture stage. In this article we investigated the cause of mass mortalities occurring 13-20 dpost-hatching in 3 cycles of cobia larviculture. Wet mounts from diseased larvae gills revealed thepresence of cyst-like basophilic inclusions. DNA from the cysts was extracted and PCR amplifiedusing the 16S rRNA gene universal primers for prokaryotes. The amplified products weresequenced and analyzed using BLAST, finding a similarity of 99% with Endozoicomonas elysicola,a Gram-negative bacterium. Confirmation of E. elysicola was conducted by designing a specificprobe for in situ hybridization. Specific primers were also designed for diagnostic purposes. This isthe first report of epitheliocystis in cobia larvae and also the first report of E. elysicola as anepitheliocystis-causing agent.102. Mendoza-Franco, E.F. & Vidal-Martinez, V.M. (2011). First records of known endoparasiticspecies of Pseudempleurosoma Yamaguti, 1965 (Monogenoidea: Dactylogyridae) fromTetraodontid and Rachycentrid fish off the Northern coast of the Yucatan Peninsula, Mexico.Journal of Parasitology, 97(6): 1020-1025.Monogenoideans infecting the rectum of the wild checkered puffer fish, Sphoeroides testudineus(Tetraodontidae), and the pyloric ceca of the cultured cobia, <strong>Rachycentron</strong> <strong>canadum</strong>(Rachycentridae), from the northern coast of the Yucatan Peninsula, Mexico, were morphologicallyidentified as Pseudempleurosoma carangis Yamaguti, 1965 and Pseudempleurosoma gibsoniSantos, Mourão and Cárdenas, 2001 (Dactylogyridae), respectively. Morphometric comparisonbetween the paratypes of P. carangis and those from S. testudineus showed that the latter differonly in the length of the body, germarium, and dorsal anchors. Similarly, a small form of P. gibsonibased on body size was detected in the present study. These metric differences may be attributableto the host effect, i.e., S. testudineus / R. <strong>canadum</strong> versus Caranx lugubris (Carangidae) (type hostof P. carangis) from Hawaii and Paralonchurus brasiliensis (Sciaenidae) (type host of P. gibsoni)from Brazil, or by the degree of maturity, or both. In view of these considerations, new illustrationsand several supplemental observations for P. carangis and P. gibsoni are provided. The presentfindings also represent new geographical records, and new sites of infection, e.g., rectum andpyloric ceca, for species of Pseudempleurosoma, and the first known endoparasiticmonogenoideans infecting tetraodontid and rachycentrid fishes in Mexico.103. Moravec, F. & De Buron, I. (2009). Two new species of Philometrids (Nematoda: Philometridae)from marine fishes off South Carolina. Journal of Parasitology, 95(3), 722-727.Two new species of philometrid nematodes, Philometra gymnothoracis n. sp. and Philometroidesmarinus n. sp., are described from female specimens collected from the body cavity of the spottedmoray, Gymnothorax moringa (Cuvier) (Muraenidae, Anguilliformes), and the cobia, <strong>Rachycentron</strong><strong>canadum</strong> (Linnaeus) (Rachycentridae, Perciformes), respectively, from off the Atlantic coast ofSouth Carolina. Philometra gymnothoracis n. sp. is mainly characterized by the conspicuouslydepressed mouth, the presence of 8 small cephalic papillae arranged in 4 submedian pairs, theesophagus with an anterior bulbous inflation, 2 small papilla-like caudal projections, the bodylength of the gravid female 435-760 mm, short ovaries, the length of larvae from the uterus 474-544µm, and by the location in the host (body cavity). Philometroides marinus n. sp. differs from itscongeners parasitizing marine and brackish water fishes mainly in having small cuticular bossesonly on the anterior part of the body; in possessing 4 markedly large cephalic projections, each with2 minute papillae, 2 large caudal projections, and in the location in the host (body cavity); the bodylength of subgravid and gravid females is 130-550 mm and that of larvae from the uterus 600-642µm104. Nahhas, F.M. & Sey, O. (2002). Digenetic trematodes from marine fishes off the coast of Kuwait,Arabian Gulf: Superfamily Hemiuroidea. Acta Zoologica Academiae Scientiarum Hungaricae,48(1), 1-20.30
- Page 1 and 2: COBIA (Rachycentron canadum)A SELEC
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- Page 8 and 9: 15. Darden, T.L., Walker, M.J., Bre
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- Page 12 and 13: 30. Franks, J.S., Warren, J.R. & Bu
- Page 14 and 15: 37. Hou, Y., Feng, J., Ning, Z., Ma
- Page 16 and 17: Cobia, Rachycentron canadum, is an
- Page 18 and 19: The spawning season, late June thro
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- Page 24 and 25: showed that cobia fed the diet cont
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- Page 28 and 29: 83. George, P.V. & Nadakal, A.M. (1
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- Page 40 and 41: enefit the rural poor, whereas offs
- Page 42 and 43: government, and research institutes
- Page 44 and 45: 137. Kaiser, J.B. & Holt, G.J. (200
- Page 46 and 47: (DHA) and vitamin E levels compared
- Page 48 and 49: and simplify water management. In t
- Page 50 and 51: growth rates (SGR) did not exceed t
- Page 52 and 53: this paper. ANOVA showed that food
- Page 54 and 55: 170. Weirich, C.R., Stokes, A.D., S
- Page 56 and 57: fingerlings for grow-out. This stud
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- Page 60 and 61: 190. Zhang, H., Mao, L., Feng, J.,
- Page 62 and 63: This suggests that the enrichment o
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- Page 66 and 67: 208. Weirich, C.R., Stokes, A.D., S
- Page 68 and 69: trypsin activities of intestine of
- Page 70 and 71: decreased gradually as fish body we
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- Page 74 and 75: cobia were 21.72 mg kg -1 , 22.38 m
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- Page 78 and 79: than the optimal requirement of cob
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soybean meal in Cobia, Rachycentron
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261. Sun, L., Chen, H., Huang, L.,
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fishes and invertebrates. Here we i
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274. Watson, A.M., Buentello, A. &
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estimated to be 44.7 mg kg -1 based
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20% of alternative protein meal, ne
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levels of methionine (0.61%, 0.83%,
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298. Mach, D.T.N. & Nortvedt, R. (2
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acids (FFA), peroxide value (PV), t
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068Breitenbach, B.078Brenkert, K.01
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271Duncan, M.226Dung, L.Q.023DuPaul
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139, 149Kilduff, P.180Kim, I.H.088K
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265Myrseth, B.140Nabavi, S.M.B.001,
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244Shi, C.071Shi, G.218, 262, 287Sh
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291Xie, J.269, 270Xu, H.037, 190Xu,