Annual Scientific Report

Cytometry and cell sorting
www.idibaps.ub.edu/citomica
Flow cytometry measures and analyzes the characteristics of single particles, normally cells, as they move in a stream and are
passed through a laser. Thousands of cells can be analyzed by a flow cytometer in a single second. It is possible to mark the
particles with one or more fluorescent substances that then makes it possible to measure physical characteristics such as cell
size, shape, internal complexity and any cell component or function that can be detected by a fluorescent compound.
The applications of flow cytometry are numerous, and this has led to the widespread use of these instruments in the biological
and medical fields.
The facility is subdivided into four groups: FACS analysis, data analysis, cell sorting and high resolution images of each cell
directly in flow.
1. Analysis of population of interest (Up to 18 colors)
• FACSCalibur (Up to 4 colors)
• FACSCantoI (Up to 6 colors)
• FACSCantoII (Up to 8 colors)
• LSRFortessa SORP HTS 4 LASER (Up to 16 colors)
• LSRFortessa SORP 5 LASER (Up to 18 colors)
2. Apoptosi
• FACSCalibur
• FACSCantoI
• FACSCantoII
• LSRFortessa SORP HTS 4 LASER
• LSRFortessa SORP 5 LASER
3. Cell cycles
• FACSCalibur
• FACSCantoI
• FACSCantoII
• LSRFortessa SORP HTS 4 LASER
• LSRFortessa SORP 5 LASER
4. Functional Analysis
• FACSCalibur
• FACSCantoI
• FACSCantoII
• LSRFortessa SORP HTS 4 LASER
• LSRFortessa SORP 5 LASER
5. Stem Cells
• LSRFortessa SORP 5 LASER
6. Viability
• FACSCalibur
• FACSCantoI
• FACSCantoII
• LSRFortessa SORP HTS 4 LASER
• LSRFortessa SORP 5 LASER
40