Annual Scientific Report
AReA 1 - IDIBAPS
AReA 1 - IDIBAPS
- No tags were found...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
SOCIETY<br />
Electronic Microscopy Unit (UB)<br />
Services<br />
Transmission electron microscopy (TEM)<br />
• Ultrastructure studies of tissues and cell cultures (on different subtrates)<br />
• Studies of suspensions ( bacteria, virus, flagella, lipid vesicles...)<br />
• Studies of cellular organelles extraction (reticulum, mitochondria, exosomes...)<br />
• Molecular localization and colocalization of proteins, glucidic residues,...<br />
• Molecular localization of DNA or RNA sequences by “in situ” hybridation<br />
• Cytochemical studies (enzymatic activity, enzymatic digestions...)<br />
• Correlative techniques (optical, confocal and electron microscope)<br />
• Preparing of samples for TEM-based microanalysis<br />
• Studies of viruses, organules and proteins by negative staining<br />
Scanning electron microscopy (SEM)<br />
• Microstructural analysis of surface cell cultures on different substrates (glass, thermanox, aclar,...)<br />
• Surface characterization of tissues or organs<br />
• Studies of cellular suspensions(bacteira<br />
• Studies of surfaces: bony, teeth, cornea, skin,...<br />
• Characterization of biofilms and their formation<br />
• Morphological studies of small organisms and micro-organisms<br />
• Surface molecular localization<br />
• Characterization of biomaterials, implants...<br />
• Study, analysis and assessment of the images<br />
Examples of applications<br />
• Biomedical characterization of any kind of cell culture or tissues by SEM or TEM<br />
• Studies of markers incorporation (colloidal gold, quantum-dots..) inside the tissues or cell culture (ex. Inhalation of nanogold<br />
and their localisation by light and transmissionn electron microscopy)<br />
• Sperm cell study by TEM and SEM for diagnosis<br />
• Studies of biofilms on water filters, tracheal tubes, historical monuments...<br />
• Characterization of protein aggregates for example in Alzheimer’s disease<br />
• Biopsies in pathology<br />
• Calcium microanalysis detection<br />
Sample preparation types<br />
• Cryomethods preparation (cryofixation)<br />
- High pressure cryofixation, cryosubstitution, cryo embedding in epoxy resins ( for ultrastructural studies)<br />
- High pressure cryofixation, cryosubstitution, cryo embedding in acrylic resins at low temperatures (molecular location studies:<br />
immunolocalization, “in situ” hybridization, etc.)<br />
- High pressure cryofixation, cryosubstitution, critical point process (3-D studies using scanning electron microscopy)<br />
• Standard preparation method (chemical fixation)<br />
- Chemical fixation, dehydration and embedding in epoxy resins for ultrastructural studies<br />
- Ultramicrotomy at room temperature<br />
- Negative staining (viruses, flagella, proteins, etc.)<br />
- Cytochemical techniques (detection of enzymes, glycoproteins, sugars, etc.)<br />
- Chemical fixation, dehydration, critical point (scanning electron microscopy)<br />
48
Change language