Annual Scientific Report

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SOCIETY DNA Unit (Hospital Clínic) Services • DNA extraction • DNA sequencing • Genotyping (Analysis of DNA fragments, microsatellites, SNPs, MLPA, etc.) Equipment • One DNA capillary sequencer (IDIBAPS) • One ultra-deep sequencing system (HC) • Two genetic analyzers (HC/IDIBAPS) • Two automatic DNA extraction systems (HC) <strong>Scientific</strong> coordinator(s): • Dr. Joan Clària HC jclaria@clinic.ub.es Staff: • Ms. Montserrat Bernat Specialist technician (HC) • Ms. Anabel Martínez Specialist technician (IDIBAPS) Location: • Hospital Clinic’s Biological Diagnosis Centre Stairway 7, 5th floor. HC (~20 sq m) 46
IDIBAPS Research and Innovation Confocal microscopy unit (SCT-UB) Services Light microscopy • Confocal laser microscopy • Two-photon excitation microscope (multiphoton) • Wide-field microscopy Light microscopy applications • Fluorescent, reflection and transmitted light, multi-channel and multidimensional (xyztλ) images. Mosaic and multi-position images • Live cell microscopy • Molecular proximity analysis: FRET (Fluorescence resonance energy transfer) • Molecular dynamics analysis by photobleaching and photolabelling techniques: - FRAP (Fluorescence recovery after photo-bleaching) - Inversed FRAP - FLIP (Fluorescence loss in photo-bleaching) - Photo-activation, photo-conversion • Intravital Microscopy (live animal): - Confocal, multi-photon - Resonant scan (high speed) • Two photon excitation microscopy (Multi-photon): - Fluorescence - Second Harmonic Generation Cell culture and micromanipulation • Maintenance of cell lines for microscopy experiments • Microinjection • Molecular and cellular dyes (transfection, staining) Equipment • Multiphoton and high-velocity spectral confocal microscope (Leica TCS-SP5) • Spectral confocal microscope (Leica TCS-SL) • Confocal microscope (Leica TCS-NT) • Microinjector (Leica AS TP) • Wide-field fluorescence microscope (Leica DMI6000 B) (Faculty of Medicine) • General laboratory • Culture room • Imaging analysis workstations <strong>Scientific</strong> coordinator(s): • Dr. Carles Enrich Department of Cellular Biology, UB enrich@ub.edu Staff: • Dr. Maria Calvo Senior technician and head of the Confocal Microscopy Unit, IDIBAPS mcalvo@sct.ub.es • Anna Bosch Specialised technician, IDIBAPS abosch@sct.ub.es Location: • Faculty of Medecine, South Wing, 5 floor (c/ Casanova, 143, 08036, Barcelona) Image processing and analysis • Technical assistance in image processing and analysis (3D reconstruction, quantification, co-localization, FRET analysis, molecular dynamics analysis, particle tracking) • Development of custom instructions macros • Training days of image processing and analysis User support and innovation • Assistance in planning, preparation, performance and analysis of light microscopy experiments • Training in the use of equipment • Development of new methodologies 47
Page 3 and 4: Annual Scientific Report Rosselló,
Page 5: INDEX Foreword IDIBAPS • Introduc
Page 8 and 9: Presentation IDIBAPS Foreword Intro
Page 10 and 11: Organisation chart Governing Board
Page 12 and 13: INNOVATION IDIBAPS Organisation cha
Page 14 and 15: It should be mentioned that the sum
Page 16 and 17: Research resources The funding obta
Page 18 and 19: Institució Catalana de Recerca i E
Page 20 and 21: Innovation and transference With th
Page 22 and 23: Spin-off companies The knowledge de
Page 24 and 25: Impact upon clinical practice The f
Page 26 and 27: Title Associated Journal Short Text
Page 28 and 29: IDIBAPS IN FIGURES Surgical innovat
Page 30 and 31: Study of experimental surgery in la
Page 32 and 33: Abreviated Journal Name Original Ar
Page 34 and 35: Abreviated Journal Name Original Ar
Page 36 and 37: INTRODUCTION IDIBAPS Platforms Biob
Page 38 and 39: People Scientific coordinator(s): D
Page 40 and 41: Cytometry and cell sorting www.idib
Page 42 and 43: SOCIETY Functional Genomics The Gen
Page 44 and 45: INTRODUCTION Shared Platforms Prote
Page 48 and 49: SOCIETY Electronic Microscopy Unit
Page 50 and 51: NETWORKING Spanish excellence netwo
Page 52 and 53: Spanish Biomedical Research Centre
Page 54 and 55: AIDS Research Network (RIS) www.ret
Page 56 and 57: Networked Biomedical Research Suppo
Page 58 and 59: CIBER IDIBAPS Team Principal invest
Page 60 and 61: CENITS AND CONSOLIDERS CENITs and C
Page 62 and 63: NoE - Genetic and environmental ris
Page 64 and 65: TRAINING Research seminars The rese
Page 66 and 67: Author Title Doctorate thesis reade
Page 68 and 69: IDIBAPS and society This research c
Page 71 and 72: Inflammatory joint diseases 72 Syst
Page 73 and 74: AREA 1 BIOLOGICAL AGGRESSION AND RE
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AREA 1 BIOLOGICAL AGGRESSION AND RE
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Respiratory, cardiovascular, renal
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AREA 2 Respiratory, cardiovascular,
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AREA 2 Respiratory, cardiovascular
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Liver, digestive system metabolism
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AREA 3 Liver, digestive system and
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AREA 3 LIVER, DIGESTIVE SYSTEM AND
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Clinical and experimental neuroscie
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AREA 4 Clinical and experimental ne
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oncology and HAEMATOLOGY Biological
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AREA 5 Oncology and haematology Bio
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AREA 5 Oncology and haematology Hum
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AREA 5 Oncology and haematology Dia
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AREA 5 Oncology and haematology Dia
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AREA 5 Oncology and haematology Mol
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AREA 5 Oncology and haematology Gen
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AREA 5 Oncology and haematology Mel
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AREA 5 Oncology and haematology Mel
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AREA 5 Oncology and haematology Hem
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AREA 5 Oncology and haematology Phy
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AREA 5 Oncology and haematology Phy
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AREA 5 Oncology and haematology Cel
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AREA 5 Oncology and haematology Cel
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transversal research groups Barcelo
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transversal research groups Clinica
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transversal research groups Clinica
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transversal research groups Transve
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transversal research groups Transve
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transversal research groups Researc
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Team leader index A Àlvar Agustí.
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Annual Scientific Report

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