Research Journal of Science & IT Management - RJSITM - The ...

e due to: Oral contraceptives, Obstructive pancreatitis, Hepatitis/Mononucleosis/CMV, Congestive
heart failure, Parasites etc.
MATERIAL AND METHODS
The experimental study consisted of 100 pregnant women, which were divided into groups as follows:
According to fetus age:
- 3 women in first trimester
- 19 women in second trimester
- 78 women in third trimester
According to maternal age:
- 5 women < 20 years old
- 67 women 20-30 years old
- 28 women >30 years old
According to zinc measurements:
- 64 cases were anemic
- 36 cases were normal
According to alkaline phosphatase (ALP) determination:
- 20 cases with low ALP levels
- 65 cases with normal ALP levels
- 15 cases with high ALP levels
According to maternal diagnosis:
- 34 cases were cephalic
- 14 cases with anomalies
- 5 cases with anemia
- 13 cases with preeclampsia
- 12 cases premature delivery
- 2 cases hyperemesis
- 2 cases with diabetes
- 2 cases abortion
- 2 cases illegal
- 3 cases membrane ruptures
- 3 manual rupture
- 3 cases breech delivery
- 1 case with fetal hypotrophy
- 1 case placenta previa
- 2 cases twin pregnancy
- 1 case baby death
The techniques used for the determination of serum zinc, included Colorimetry, Polarography, X-ray
fluorescence, Fluorometry and Atomic Absorption Spectroscopy (AAS). AAS techniques are preferred
in the clinical laboratory, because of their specificity, sensitivity, precision, simplicity, and relatively
low cost per analysis. (4)
The direct dilution method presented here requires less than 2 min per sample. We used Glycerol as a
solvent for the standards, and it also serves as an ideal additive for adjusting the viscosity and flow rate
of the standards. We took 2 ml blood from each pregnant woman and serum zinc level was measured
directly by using Atomic Absorption Spectrometry (VARIAN AAS-220), at the same time we
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