JIOMICS

JIOMICS | VOL 5 | ISSUE 2 | DECEMBER 2015 | 1-62
JOURNAL OF INTEGRATED OMICS
Journal of Integrated Omics
A METHODOLOGICAL JOURNAL
HTTP://WWW.JIOMICS.COM
Special Issue: Proceeding Abstracts of the 4 th International Congress on Analytical Proteomics (ICAP 2015)
Development and evaluation of method for metalloproteins detection
involved in bipolar disorder
G. S Pessôa* 1,2 , J. R. de Jesus 1,2 , A. Sussulini 1,2 , J. L. C. Martínez 3 ; M. A. Z. Arruda 1,2
1
Spectrometry, Sample Preparation, Mecanization Group, Analytical Chemistry Department, Institute of Chemistry, University of Campinas,
P.O. box 6154, 13083970, Campinas, SP, Brazil. 2 National Institute of Science and Technology for Bioanalytics, Analytical Chemistry
Department, Institute of Chemistry, University of Campinas, P.O. box 6154, 13083970, Campinas, SP, Brazil. 3 Bioscope group. Chemistry
Department. Faculty of Sciences and Technology. New University of Lisbon. Campus de Caparica,. 2829-516. Caparica. Portugal.
*Corresponding author: gpessoa@iqm.unicamp.br
Available Online: 31 December 2015
Abstract
Purpose: Bipolar disorder (BD) is characterized as a chronic psychiatric illness, severe and highly debilitating, with recurrent mood disorders,
presenting episodes of mania and depression separated by periods of normal behavior (Sussulini, et al., 2009;).The cause of bipolar
disorder is unknown, although it is known that a variety of biochemical, genetic, and environmental factors can be involved in the process of
developing of the disorder. To date, there is no biochemical test to confirm the disease, and the diagnosis is still based on experience and clinical
judgment (Jesus, et al., 2015). The main focus of this work was to propose a metalloproteomic strategy for BD, since this area has been not
carried out in the literature.
Experimental description: Metal and metalloid ion involved in this pathology were determined by ICP-MS, using a dynamic reaction cell
(Perkin Elmer, ELAN DRC-e). For metalloproteomic evaluation, a mild procedure for removal of major abundant protein was applied. For
this task, a high performance liquid chromatograph was used to separate the proteins, using a sequential detection of proteins (UV-Vis) and
metal ions (hyphenated LC-ICP-MS).
Results: Considering the elemental determination, the DRC strategy was considered as an efficient method in the removal of interferences,
since satisfactory results in terms of trueness and precision were obtained, using a certified reference material. Thus, an ionomic profile
of each group was possible. After the chromatographic separation of proteins and metalloproteins, the removal of major abundant proteins is a
main challenging step, since for a metallomic study, the information about tertiary structure must be preserved as intact as possible. The detection
of proteins in UV-Vis and metalloproteins in LC-ICP-MS confirmed that our strategy was reliable for a metalloprotemic evaluation.
Conclusions: Differential levels of metal and metalloid ions were observed when comparing the three studied groups, as well as the detection
of metalloproteins by LC-ICP-MS. Thus, our method can be considered as reliable for evaluation of BD disorder.
Keywords: bipolar disorder, metallomic, metalloprotein.
Acknowledgements: FAPESP, FAEPEX, Capes and CNPq
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