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JIOMICS | VOL 5 | ISSUE 2 | DECEMBER 2015 | 1-62 JOURNAL OF INTEGRATED OMICS Journal of Integrated Omics A METHODOLOGICAL JOURNAL HTTP://WWW.JIOMICS.COM Special Issue: Proceeding Abstracts of the 4 th International Congress on Analytical Proteomics (ICAP 2015) Comparative proteomic profiling of brain tissues of rat with different behavioral characteristics during emotional stress. N.E. Sharanova*, N.V. Kirbaeva Institute of Nutrition, Moscow, Russia. *Corresponding author: sharanova@ion.ru Available Online: 31 December 2015 Abstract Purpose: Evidence from animal studies has demonstrated that the limbico-reticular complex is initially involved in emotional responses. The amygdale and hippocampus are the most multifunctional structures of the limbic system. The reticular formation is directly connected with the emotions due to promote new and significant information to the different brain regions. To identify the differences of proteomic expression in rat brain caused by emotional stress we performed comparative proteomic analysis. Experimental description: Wistar male rats were divided on 2 groups of behaviorally passive and active animals. Immobilization of rats during 12h (from 9p.m. till 9a.m.) served as a model of acute emotional stress. The protein expression profiles of hippocampus, amygdale, reticular formation and cortex were studied using by two-dimensional electrophoresis and MALDI-TOF. Results: The proteomic analysis showed the down-regulated expression of GFAP, septine-5, calreticulin, alpha-synuclein, phosphatidylethanolamine-binding protein 1, thiomorpholine-carboxylate dehydrogenase, prohibitin, glutamine synthetase and up-regulated expression of lactate dehydrogenase, F-actin-capping protein subunit alpha-2 in different parts of brain depending on behavioral type of rats and the stage of stress. Conclusions: Thus, individual behavioral features affect the specific pathway of organism response to the stress and determine an adaptive potential of the organism. Keywords: brain, emotional stress, comparative proteomics, behavior Acknowledgements: Prof. S.Pertsov from P.K.Anokhin Research Institute of Normal Physiology, Moscow, Russia. 1-62: 29
JIOMICS | VOL 5 | ISSUE 2 | DECEMBER 2015 | 1-62 JOURNAL OF INTEGRATED OMICS Journal of Integrated Omics A METHODOLOGICAL JOURNAL HTTP://WWW.JIOMICS.COM Special Issue: Proceeding Abstracts of the 4 th International Congress on Analytical Proteomics (ICAP 2015) Cell-free lysates for the production of difficult proteins F. Bernhard* 1 , D. Foshag 2 , S. Rupp 3 , Ernst Thuer 4 , V. Dötsch 1 , E. Henrich 1 , R. Rues 1 1 Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, J.W. Goethe-University, Frankfurt-am-Main, Germany. 2 Institute for Interfacial Engineering and Plasma Technology IGVP, University of Stuttgart, Stuttgart, Germany. 3 Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany. 4 Comparative Genomics Group, CRG-Centre for Genomic Regulation, Barcelona, Spain. *Corresponding author: fbern@bpc.uni-frankfurt.de Available Online: 31 December 2015 Abstract Purpose: Cell-free production of proteins and other biomolecules has emerged during the last decade as an important core technology in the new field of synthetic biology. Lysates of either eukaryotic or prokaryotic organisms can be employed for the synthesis of particularly difficult proteins such as membrane proteins, large multisubunit assemblies or highly oxidized proteins. Cell-free expression provides an open and accessible reaction environment. Additionally, functional properties of the lysate proteomes can easily be modulated in order to generate ideal conditions for the production of high quality samples of individual target proteins. Experimental description: Protocols for the preparation of cell-free lysates have been developed and optimized. Libraries of additives have been screened for the manipulation and optimization of lysate proteomes. Results: We present strategies for the manipulation of cell-free lysates and reaction conditions for the efficient production of difficult proteins such as G-protein coupled receptors or enzymes. We further exemplify the optimization of protein sample quality by directed fractionation of lysate proteomes and selective inactivation of particular enzyme classes and pathways. Examples focusing on the structural and functional characterization of membrane proteins will be discussed. Conclusions: Lysate proteomes for the cell-free production of biomolecules can be adjusted according to the requirements of individual targets. Keywords: cell-free expression, bacterial lysates, membrane protein environments, synthetic biology, artificial proteomes. Acknowledgements: The work was funded by the Collaborative Research Center (SFB) 807 of the German Research Foundation (DFG) and by the German Ministry of Education and Science (BMBF). Support was further obtained by Instruct, part of the European Strategy Forum on Research Infrastructures (ESFRI). 1-62: 30
Page 1 and 2: Volume 5 | Issue 2 | December 2015
Page 3 and 4: Randen Patterson Center for Computa
Page 5 and 6: Biodiversity Research Center, Acade
Page 7 and 8: Belgium Bart De Spiegeleer Ghent Un
Page 9 and 10: Instituto Superior Técnico, Centro
Page 11 and 12: Veronica Mainini Dept. Health Scien
Page 13: Xuequn Chen Department of Molecular
Page 16 and 17: Committees Conference Chairs 4 th I
Page 18 and 19: CONTENTS OF VOLUME 5 | ISSUE 2 | DE
Page 20 and 21: JIOMICS | VOL 5 | ISSUE 2 | DECEMBE

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