JIOMICS

JIOMICS | VOL 5 | ISSUE 2 | DECEMBER 2015 | 1-62
JOURNAL OF INTEGRATED OMICS
Journal of Integrated Omics
A METHODOLOGICAL JOURNAL
HTTP://WWW.JIOMICS.COM
Special Issue: Proceeding Abstracts of the 4 th International Congress on Analytical Proteomics (ICAP 2015)
The quantitative comparison of the human tooth pulp obtained from cariesfree
and caries-susceptible people
M. Jágr*, A. Eckhardt, S. Pataridis, L. Kulhavá, I. Mikšík
Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14220, Prague 4, Czech Republic *Corresponding author:
jagr@biomed.cas.cz
Available Online: 31 December 2015
Abstract
Purpose: The majority of the human population worldwide experiences dental caries. Only a minor part of the population (ca. 10 % of
people in the age at 30 years) is resistant to development of this oral disorder. The anti-caries resistance can be caused by overall immune system,
nourishment, dental care efficiency and/or by many other causes, such as composition of saliva or teeth. Differences in the abundances of
the responsible proteins in these tissues between caries-resistant and caries-susceptible people are expected and are in the center of our interest.
The aim of our work was to investigate the proteomic profile of human tooth pulp from adult people and compare them mutually.
Experimental description: Sound human teeth extracted for clinical reasons from adult patients (aged 17-40) were divided into two groups
with respect to the number of dental caries (caries-susceptible vs. caries-resistant people). The proteins from individual tooth pulp samples
were extracted and processed by the method described previously [1]. The proteins were separated either by two-dimensional electrophoresis
followed by Coomassie colloidal blue staining or by difference gel electrophoresis and the resulting protein maps were quantitatively evaluated.
Spots exhibiting statistically significant changes were excised, digested by trypsin, and analyzed by nano-liquid chromatography coupled to a
MaXis Q-TOF mass spectrometer with ultrahigh resolution.
Results: We detected more than several hundreds of spots in the proteome maps of each human tooth pulp sample. Individual proteome
maps were compared by both methods (2-DE and DIGE) and changes in the protein abundances were identified among the individuals. The
observed differences were statistically evaluated to find the significant changes. Some of these spots corresponded e.g. to peroxiredoxin 1, GTPbinding
nuclear protein Ran, apolipoprotein A-I, glutathione S-transferase P, phosphoglycerate kinase 1 or retinal dehydrogenase. These proteins
exhibits variety of biological functions: cell communication and signal transduction, transport or metabolism.
Conclusions: We determined some quantitative differences in the whole proteomes of human teeth pulp samples between cariousresistant
and carious-susceptible people. Special emphasis will be engaged in further investigations of these proteins that may be involved in
formation of dental caries due to their biological functions.
Keywords: human tooth pulp, proteome, two-dimensional gel electrophoresis.
Acknowledgements: This work was supported by the Ministry of Health Departmental Program for Research and Development
(NT14324-3/2013), and with support for long-term conceptual development of research organization RVO:67985823.
References:
[1] A. Eckhardt, M. Jágr, S. Pataridis, I. Mikšík, J. Endodont. 40 (2014) 1961-1966.
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