JIOMICS

JIOMICS | VOL 5 | ISSUE 2 | DECEMBER 2015 | 1-62
JOURNAL OF INTEGRATED OMICS
Journal of Integrated Omics
A METHODOLOGICAL JOURNAL
HTTP://WWW.JIOMICS.COM
Special Issue: Proceeding Abstracts of the 4 th International Congress on Analytical Proteomics (ICAP 2015)
Some proteins of possible -clinical interest found in samples of human
intestinal mucus (HIM)
Gómez-Buitrago Paola-Andrea* 1 , González-Correa Carlos-Augusto 1 , Taborda-Ocampo Gonzalo 1 , Acuña-Sornoza Ricardo 2 ,
Santacoloma-Osorio Mario 3 , Zezzi-Arruda Marco-Aurelio 4 .
1
Research Group on Electrical Bio-Impedance, Doctoral Program on Biomedical Sciences, University of Caldas, Manizales, Colombia.
2
Colombian National Coffee Research Center CENICAFE, Chinchiná, Colombia. 3 linical Department, University of Caldas, Manizales,
Colombia. 4 State University of Campinas, Institute of Chemistry, Campinas, Brazil. *Corresponding author: quimicapao@gmail.com
Available Online: 31 December 2015
Abstract
Purpose: To report the identification of some proteins isolated from samples of human intestinal mucus (HIM) with possible clinical
interest.
Experimental description: HIM was collected from six volunteers admitted to a leaning program which included a colon cleansing protocol
and a rectosigmoidoscopy. Protein extraction was achieved by a denaturalization buffer, separation was performed by gel electrophoresis
both with SDS-PAGE and 2D-PAGE. Protein shotgun was done with solution desalinization by SPE and albumin depletion. Digested peptides
were analysed using a NanoAcquity UPLC cromatograph coupled to a HDSM espectrometer, equiped with a NanoESI source. Samples were
injected to a C 18 precolumn with a flow of 5 μL/min water/acetonitrile (97:3) with 0,1% formica cid and then transfered to an analytical column
C 18 and eluted with a flow of 1 μL/min.
Partial results: 1. A methodology for the analysis of HIM from a single person is reported, from the preparation of the patients for the
collection of the sample, till protein characterization of the mucus. This is done using mass spectrometry, using three different techniques: SDS
-PAGE, 2D-PAGE y shotgun; 2. 14 proteins of clinical interest have been identified so far: Annexin A2, Calcium-activated chloride channel
regulator, Chain crystal structure of human calcineurin complexed with cyclosporin A and human cyclophilin, Complement C3, Disulfide
isomerase, Galectin-3 y 4, Inmunoglobulin programmed cell death 1 ligand, LAMTOR3, Muc2, Nuclear Protein 8, RuvB like 2, Tyrosine Protein
phosphatase non receptor type 11 and Zymogen granule membrane protein 16.
Conclusion: The proteomic study of HIM can be a very useful tool for the study of different diseases, not only of those directly related to
the digestive system, bus also many associated with the immune system.
Keywords: electrophoresis, human intestinal mucus, shotgun proteomics.
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