JIOMICS

JIOMICS | VOL 5 | ISSUE 2 | DECEMBER 2015 | 1-62
JOURNAL OF INTEGRATED OMICS
Journal of Integrated Omics
A METHODOLOGICAL JOURNAL
HTTP://WWW.JIOMICS.COM
Special Issue: Proceeding Abstracts of the 4 th International Congress on Analytical Proteomics (ICAP 2015)
Differential protein expression in the testes of ganders under different
monochromatic light sources
C. Y. Cheng 1 , Z. X. Zhuang 1 , C. J. Chen 2,3 , S. C. Chang 1,4 , M. J. Lin 1,4 , T. Y. Lin 4 , Y. S. Jea 4 , H. L. Chan 5,6 , S. Y. Huang* 1,7,8,9
1
Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan. 2 Proteomics Core Laboratory, Department of
Medical Research, China Medical University Hospital, Taichung 40402, Taiwan. 3 Graduate Institutes of Integrated Medicine, China Medical
University, Taichung 40402, Taiwan. 4 Changhua Animal Propagation Station, Livestock Research Institute, Council of Agriculture, Taichung
52146, Taiwan. 5 Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu 30013, Taiwan. 6 Department of
Medical Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan. 7 Agricultural Biotechnology Center, National Chung Hsing
University, Taichung 40227, Taiwan. 8 Center for the Integrative and Evolutionary Galliformes Genomics, iEGG Center, National Chung Hsing
University, Taichung 40227, Taiwan. 9 Center of Nanoscience and Nanotechnology, National Chung Hsing University, Taichung 40227,
Taiwan. *Corresponding author: syhuang@dragon.nchu.edu.tw
Available Online: 31 December 2015
Abstract
Purpose: In modern poultry production, patterns of artificial illumination treatment such as light schedule, intensity, and color are important
factors that influence productive performances. The purpose of this study was to investigate whether different monochromatic light
sources affect the protein expression in the testes of ganders.
Experimental description: Six male White Roman ganders were used. The ganders were allocated into white or blue light groups. The
lighting photoperiod of 7L:17D was applied for 6 weeks as an adjusting period. After the adjusting period, the photoperiod was changed to
9L:15D and maintained for 24 weeks. The ganders were sacrificed at the end of treatment to collect testis samples for proteomic analysis.
Results: The results of two-dimensional difference gel electrophoresis revealed that 37 protein spots were differentially expressed in the testes
of ganders with different monochromatic light treatments. A total of 31 spots were identified by MALDI-TOF/TOF. Gene ontology analysis
revealed that the differentially expressed proteins were mostly located in extracellular vesicular exosome (27%, 7 proteins), nucleus (11%, 3
proteins), and cytoplasm (11%, 3 proteins). Most of the proteins were with molecular function of protein binding (23%, 6 proteins), ion binding
(23%, 6 proteins), carbohydrate binding (19%, 5 proteins), and heterocyclic compound binding (15%, 4 proteins). The majority of the proteins
were participated in biological process of biological regulation (27%, 7 proteins) and cellular process (23%, 6 proteins). In addition, proteins
related to sperm maturation and capacitation, including hemoglobin, apolipoprotein A-1, and phosphatidylethanolamine-binding protein
1, were downregulated after blue light treatment. The result suggested that blue light might disturb sperm functions and fertility on ganders.
Conclusions: A total of 37 protein spots were differentially expressed in the testes of ganders with blue and white light exposure. Proteins
related to sperm maturation and capacitation were downregulated in the testes of blue light treated ganders. The exact role of the differentially
expressed proteins needs further investigation.
Keywords: Testes, Monochromatic light sources, Protein expression, Ganders.
Acknowledgements: The authors would like to thank the Council of Agriculture (Contract No. 102AS-2.1.5-L1-L2) and Ministry of Education
(under the ATU plan), Taiwan for financial support of this study.
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