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Evaluation of Bioassays and Wastewater Quality ... - Oekotoxzentrum

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Biological <strong>and</strong> powdered activated carbon –UF treatment<br />

Treatment <strong>of</strong> the wastewater with PAC-UF led to an additional 61 to 100 % elimination <strong>of</strong> the<br />

remaining specifically-acting substances when compared to the removal in the biological<br />

treatment (Figure 5).<br />

Green algae: photosynthesis inhibition<br />

Green Green algae: grow th inhibition<br />

YES YES assay: assay: estrogenic estrogenic effects effects<br />

ER ER CALUX: CALUX: estrogenic estrogenic effects effects<br />

AR AR CALUX: CALUX: <strong>and</strong>rogenic <strong>and</strong>rogenic effects<br />

GR GR CALUX: CALUX: glucocorticoid like effects<br />

PR PR CALUX: CALUX: progesterone progesterone like effects effects<br />

PPARg1 PPARg1 CALUX CALUX<br />

-385 ± 196 %<br />

-20% 0% 20% 40% 60% 80% 100%<br />

Elimination Biological Treatment<br />

Green algae: photosynthesis inhibition<br />

Green algae: grow th inhibition<br />

YES assay: estrogenic effects<br />

ER CALUX: estrogenic effects<br />

AR CALUX: <strong>and</strong>rogenic effects<br />

GR CALUX: glucocorticoid like effects<br />

PR CALUX: progesterone like effects<br />

PPARg1 CALUX<br />

0% 20% 40% 60% 80% 100%<br />

Elimination<br />

Biological + PAC-UF Treatment<br />

Swiss Centre for Applied Ecotoxicology, Eawag/EPFL · Überl<strong>and</strong>strasse 133 · CH-8600 Dübendorf<br />

www.oekotoxzentrum.ch<br />

n<br />

n<br />

s<br />

s<br />

s<br />

s<br />

s<br />

X<br />

0% 20% 40% 60% 80% 100%<br />

Elimination PAC-UF Treatment<br />

Figure 5: Mean elimination efficiency for specific effects measured in in vitro bioassays in the different<br />

treatment steps: biological, PAC-UF <strong>and</strong> biological + PAC-UF treatment (mean ± SD <strong>of</strong> 2 nd to 4 th measurement<br />

campaign).<br />

The combination <strong>of</strong> biological <strong>and</strong> PAC-UF treatments removed all <strong>of</strong> the specifically-acting<br />

substances to more than 80 % (elimination efficiencies ranged from 92 to 100 %).<br />

26

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