Evaluation of Bioassays and Wastewater Quality ... - Oekotoxzentrum

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Table 9: Saccharomyces cerevisiae, estrogen receptor binding: Estradiol equivalent concentration, EEQ [ng/L] (SD in brackets), elimination efficiency [%] and change index (CITEQ) for the respective treatment steps. EN Entrée STEP, SB Sortie biologie (1 st MC), LF Lit fluidisé (2 rd - 4 th MC), OZ Sortie O3, CAG Sortie charbon actif granulé (1 st - 2 nd MC), SF Sortie filtre à sable (3 rd - 4 th MC), PAC-UF Sortie charbon actif en poudre - Ultrafiltration (2 nd - 4 th MC). n.m. not measured. Date Ozone dosage (mgO3 gDO C -1 ) PAC conc. (mg PAC/L wastewater) EN SB LF Elimination Biology (%) Effect Biology Swiss Centre for Applied Ecotoxicology, Eawag/EPFL · Überlandstrasse 133 · CH-8600 Dübendorf www.oekotoxzentrum.ch OZ Elimination OZ (%) Effect OZ CAG SF Elimination OZ + SF (%) Effect OZ + SF PAC- UF Elimination PAC-UF (%) Effect PAC- UF Overall Elimination Ozonation 20.-27.07.09 0.5 No PAC 48.68 12.00 75% 4.06 0.27 98% 44.87 3.08 74% 3.89 n.m. - - 99% - (4.25) (0.88) (0.09) (0.59) 30.10.-05.11.09 0.7 10 (Norit) 100.55 0.68 99% 147.04 0.10 85% 6.53 11.43 -1571% 0.06 0.29 58% 2.35 100% 100% (12.03) (0.12) (0.05) (0.56) (0.09) 10.-17.03.10 0.8 12 (Sorbopor) 61.68 8.34 86% 7.39 0.65 92% 12.77 3.26 61% 2.56 1.32 84% 6.31 99% 98% (6.82) (0.47) (0.12) (0.33) (0.28) 25.05.-02.06.10 0.11 20 (Sorbopor) 37.15 3.03 92% 12.25 0.27 91% 11.34 0.23 92% 13.04 0.29 90% 10.32 99% 99% (2.42) (0.51) (0.01) (0.08) (0.08) Overall Elimination PAC-UF Table 10: ER CALUX, estrogen receptor binding: Estradiol equivalent concentration, EEQ [ng/L] (SD in brackets), elimination efficiency [%] and change index (CITEQ) for the respective treatment steps. LOD agonism = 0.03 ng 17ß-estradiol equivalents/l water. EN Entrée STEP, SB Sortie biologie (1 st MC), LF Lit fluidisé (2 nd - 4 th MC), OZ Sortie O3, CAG Sortie charbon actif granulé (1 st - 2 nd MC), SF Sortie filtre à sable (3 rd - 4 th MC), PAC-UF Sortie charbon actif en poudre - Ultrafiltration (2 nd - 4 th MC). n.m. not measured. Date Ozone dosage (mgO3 gDOC -1 ) PAC conc. (mg PAC/L wastewater) EN SB LF Elimination Biology (%) Effect Biology OZ Eliminatio n OZ (%) Effect OZ CAG SF Eliminatio n OZ + SF (%) Effect OZ + SF PAC -UF Elimination PAC-UF (%) Effect PAC- UF Overall Elimination Ozonation Overall Elimination PAC-UF 20.-27.07.09 0.5 No PAC 24.50 6.70 73% 3.66 0.14 98% 47.86 1.45 78% 4.62 n.m. - - 99% -
Table 11: AR CALUX, androgen receptor binding: dihydrotestosterone (DHT) equivalent concentration [ng/L] (SD in brackets), elimination efficiency [%] and change index (CITEQ) for the respective treatment steps. LOD agonism = 0.1 ng DHT equivalents/l water. EN Entrée STEP, SB Sortie biologie (1 st MC), LF Lit fluidisé (2 nd - 4 th MC), OZ Sortie O3, CAG Sortie charbon actif granulé (1 st - 2 nd MC), SF Sortie filtre à sable (3 rd - 4 th MC), PAC-UF Sortie charbon actif en poudre - Ultrafiltration (2 nd - 4 th MC). n.m. not measured. Date Ozone dosage (mgO3 gDOC -1 ) PAC conc. (mg PAC/L wastewater) EN SB LF Elimination Biology (%) Effect Biology 20.-27.07.09 0.5 No PAC 11.00 0.67 94% 16.42 (1.41) Swiss Centre for Applied Ecotoxicology, Eawag/EPFL · Überlandstrasse 133 · CH-8600 Dübendorf www.oekotoxzentrum.ch OZ < LOD Elimination OZ (%) Effect OZ - - CAG SF Elimination OZ + SF (%) 30.10.-05.11.09 0.7 10 (Norit) 4.50 0.22 95% 20.45 0.15 32% 1.47 0.67 -205% 0.33 10.-17.03.10 0.8 12 (Sorbopor) 28.00 0.48 98% 58.33 0.27 44% 1.78 0.17 65% 2.82 25.05.-02.06.10 0.11 20 (Sorbopor) 18.00 0.20 99% 90.00 0.10 50% 2.00 0.13 35% 1.54 < LOD Effect OZ + SF PAC- UF Elimination PAC-UF (%) Effect PAC- UF Overall Elimination Ozonation Overall Elimination PAC-UF Blank - - n.m. - - - -
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Evaluation of Bioassays and Wastewa
Page 3 and 4:
Note This study was carried out on
Page 5 and 6:
Main conclusions � A broad range
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9. Appendix _______________________
Page 9 and 10:
RQ Risk quotient SB Effluent biolog
Page 11 and 12: 1.3. Questions to be addressed by t
Page 13 and 14: have to be assessed thoroughly befo
Page 15 and 16: 3. Approach 3.1. Bioassays A broad
Page 17 and 18: Table 1: Overview of the applied te
Page 19 and 20: division. This event is referred to
Page 21 and 22: Samples used for in vivo bioassays,
Page 23 and 24: 4. Performance Analysis of Advanced
Page 25 and 26: 2) The CI enables a direct comparis
Page 27 and 28: Biological wastewater treatment pre
Page 29 and 30: that might be toxic substances orig
Page 31 and 32: such as a sand filter after ozonati
Page 33 and 34: 4.4. Elimination efficiency of trea
Page 35 and 36: Biological and powdered activated c
Page 37 and 38: Compared with chemical analytics, r
Page 39 and 40: 5. Evaluation of wastewater quality
Page 41 and 42: 5.2. Estrogenicity during wastewate
Page 43 and 44: 7. References Abegglen C, Escher B,
Page 45 and 46: Gracia T, Hilscherova K, Jones PD,
Page 47 and 48: Maletz S, Hollert H, 2010. Untersuc
Page 49 and 50: Stalter D, Magdeburg A, Oehlmann J,
Page 51 and 52: Figure 12: Elimination efficiencies
Page 53 and 54: Table 16: Pseudokirchneriella subca
Page 55 and 56: Classification of risk quotients fo
Page 57 and 58: Figures Biological treatment Green
Page 59 and 60: Green algae: photosynthesis inhibit
Page 61: Tables Table 7: Pseudokirchneriella
Page 65 and 66: Table 15: Vibrio fischeri luminesce
Page 67 and 68: Table 19: Pseudokirchneriella subca
Page 69 and 70: Table 23: Ceriodaphnia dubia reprod
Page 71: Table 26: Oncorhynchus mykiss survi
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