Evaluation of Bioassays and Wastewater Quality ... - Oekotoxzentrum

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Table 13: PR CALUX, progesterone receptor binding: Org-2058 equivalent concentration [ng/L] (SD in brackets), elimination efficiency [%] and change index (CITEQ) for the respective treatment steps. LOD agonism = 0.10 ng Org-2058 equivalents/l water. EN Entrée STEP, SB Sortie biologie (1 st MC), LF Lit fluidisé (2 nd - 4 th MC), OZ Sortie O3, CAG Sortie charbon actif granulé (1 st - 2 nd MC), SF Sortie filtre à sable (3 rd - 4 th MC), PAC-UF Sortie charbon actif en poudre - Ultrafiltration (2 nd - 4 th MC). n.m. not measured. Date Ozone dosage (mgO3 -1 gDOC ) PAC conc. (mg PAC/L wastewater) EN SB LF Elimination Biology (%) Effect Biology Swiss Centre for Applied Ecotoxicology, Eawag/EPFL · Überlandstrasse 133 · CH-8600 Dübendorf www.oekotoxzentrum.ch OZ Elimination OZ (%) Effect OZ CAG SF Elimination OZ + SF (%) Effect OZ + SF PAC- UF Elimination PAC-UF (%) Effect PAC- UF Overall Elimination Ozonation Overall Elimination PAC-UF 20.-27.07.09 0.5 No PAC 1.34 2.60 -95% 0.51 0.34 87% 7.65 0.37 86% 7.03 n.m. - - 75% -
Table 15: Vibrio fischeri luminescence inhibition after 30 min: EC20 [%] after 30 minutes (95% confidence interval in brackets) and change index (CI) for the respective treatment steps. EN Entrée STEP, SB Sortie biologie, LF Lit fluidisé, OZ Sortie O3, CAG Sortie charbon actif granulé, SF Sortie filtre à sable, PAC-UF Sortie charbon actif en poudre - Ultrafiltration. n.t. not toxic, n.m. not measured. If e.g. only the influent sample was toxic, a surrogate EC20 of 110% (as the “real” value is >100%) was used for the not toxic samples in order to be able to calculate a change index. Date Ozone dosage -1 (gO3 gDOC ) PAC conc. (mg PAC/L wastewater) EN SB (1 st MC) LF (2 nd - 4 th MC) Effect Biology Swiss Centre for Applied Ecotoxicology, Eawag/EPFL · Überlandstrasse 133 · CH-8600 Dübendorf www.oekotoxzentrum.ch OZ Effect OZ CAG (1 st , 2 nd MC) SF (3 rd ,4 th MC Effect OZ + SF 20.-27.07.09 0.5 No PAC 42 n.t. 2.62 n.t. - n.t. - n.m. - 30.10.-05.11.09 0.7 10 (Norit) (30.3-58.2) 100 n.t. 1.10 n.t. - n.t. - n.t. - 10.-17.03.10 0.8 12 (Sorbopor) 75.4 (60.8-93.5) n.t. 1.46 n.t. - n.t. - n.t. - 26.05.-02.06.10 0.11 20 (Sorbopor) 74.9 (50.7-93.4) n.t. 1.47 n.t. - n.t. - n.t. - Table 16: Pseudokirchneriella subcapitata growth inhibition without nutrient addition after 3 d: EC20 [%] (95% confidence interval in brackets) and change index (CI) for the respective treatment steps. EN Entrée STEP, SB Sortie biologie, LF Lit fluidisé, OZ Sortie O3, CAG Sortie charbon actif granulé, SF Sortie filtre à sable, PAC-UF Sortie charbon actif en poudre - Ultrafiltration. n.t. not toxic, n.m. not measured. If e.g. only the influent sample was toxic, a surrogate EC20 of 110% (as the “real” value is >100%) was used for the not toxic samples in order to be able to calculate a change index. Date Ozone dosage -1 (gO3 gDOC ) PAC conc. (mg PAC/L wastewater) EN SB (1 st MC) LF (2 nd - 4 th MC) Effect Biology OZ Effect OZ CAG (1 st , 2 nd MC) SF (3 rd ,4 th MC Effect OZ + SF 20.-27.07.09 0.5 No PAC 71.1 67.1 0.94 63 0.94 58.6 0.87 n.m. - (43.4-85.2) (59.2-74.3) (56.6-69.4) (54.2-62.8) 30.10.-05.11.09 0.7 10 (Norit) 39.7 32.3 0.81 38.9 1.20 47.9 1.48 32.4 1.00 (25.5-51.4) (29.2-36.2) (34.4-41.9) (43.5-53.1) (27.2-39.6) 10.-17.03.10 0.8 12 (Sorbopor) 56.38 54.57 0.97 52.21 0.96 57.16 1.05 53.7 0.98 (42.2-59.9) (51.8-57.0) (50.1-57.7) (54.4-59.7) (52.0-55.1) 26.05.-02.06.10 0.11 20 (Sorbopor) 35.23 84.23 2.39 67.82 0.81 32.38 0.38 33.23 0.39 (16.5-79.8) (70.5-93.6) (59.6-75.9) (23.1-77.6) (26.7-47.2) PAC-UF PAC-UF Effect PAC- UF Effect PAC-UF 56
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Evaluation of Bioassays and Wastewa
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Note This study was carried out on
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Main conclusions � A broad range
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9. Appendix _______________________
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RQ Risk quotient SB Effluent biolog
Page 11 and 12:
1.3. Questions to be addressed by t
Page 13 and 14: have to be assessed thoroughly befo
Page 15 and 16: 3. Approach 3.1. Bioassays A broad
Page 17 and 18: Table 1: Overview of the applied te
Page 19 and 20: division. This event is referred to
Page 21 and 22: Samples used for in vivo bioassays,
Page 23 and 24: 4. Performance Analysis of Advanced
Page 25 and 26: 2) The CI enables a direct comparis
Page 27 and 28: Biological wastewater treatment pre
Page 29 and 30: that might be toxic substances orig
Page 31 and 32: such as a sand filter after ozonati
Page 33 and 34: 4.4. Elimination efficiency of trea
Page 35 and 36: Biological and powdered activated c
Page 37 and 38: Compared with chemical analytics, r
Page 39 and 40: 5. Evaluation of wastewater quality
Page 41 and 42: 5.2. Estrogenicity during wastewate
Page 43 and 44: 7. References Abegglen C, Escher B,
Page 45 and 46: Gracia T, Hilscherova K, Jones PD,
Page 47 and 48: Maletz S, Hollert H, 2010. Untersuc
Page 49 and 50: Stalter D, Magdeburg A, Oehlmann J,
Page 51 and 52: Figure 12: Elimination efficiencies
Page 53 and 54: Table 16: Pseudokirchneriella subca
Page 55 and 56: Classification of risk quotients fo
Page 57 and 58: Figures Biological treatment Green
Page 59 and 60: Green algae: photosynthesis inhibit
Page 61 and 62: Tables Table 7: Pseudokirchneriella
Page 63: Table 11: AR CALUX, androgen recept
Page 67 and 68: Table 19: Pseudokirchneriella subca
Page 69 and 70: Table 23: Ceriodaphnia dubia reprod
Page 71: Table 26: Oncorhynchus mykiss survi
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