3DModels_for_EVs_Munich_2019_v2_IN

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Development of a 3D model for EV production and analysis Requirements for EV production: ‣ 3D environment in serum-free, or EV-free culture medium ‣ Control of cell viability, proliferation, apoptosis and necrosis ‣ High amount of EVs produced ‣ Efficient and easy recovery of EVs from the 3D cell culture ‣ Possibility to upscale the approach Requirements for modelling of tumor microenvironment ‣ Easy analysis – microscopy, immunohistochemistry ‣ Possibility of co-culture with stroma cells and other components of tumor microenvironment 12 · April 4, 2019
Application of 3DCoSeedis TM to produce and study EVs in 3D environment Models: Prostate cancer Breast cancer Gastrointestinal cancer 13 · April 4, 2019
Page 1 and 2: Studying Intercellular Communicatio
Page 3 and 4: Tumor releases into microenvironmen
Page 5 and 6: Publications frequency of studies i
Page 7 and 8: Versatile EV functions in cancer Ko
Page 9 and 10: Biogenesis of extracellular vesicle
Page 11: The need of physiological in vitro
Page 15 and 16: Application of 3DCoSeedis TM to pro
Page 17 and 18: Comparative analysis of different E
Page 19 and 20: EV populations produced under 2D an
Page 21 and 22: qNano measurements of EV120 Optipre
Page 23 and 24: To sum up ‣ The 3DCoSeedis TM are
Page 25 and 26: Studying breast cancer small EVs Ce
Page 27 and 28: Establishment of the 3D culture for
Page 29 and 30: Establishment of the 3D culture for
Page 31 and 32: Immunohistochemistry of breast canc
Page 33 and 34: Breast cancer cells produce higher
Page 35 and 36: To sum up ‣ The 3DCoSeedis TM are
Page 37 and 38: Cell spheroids and aggregates grow
Page 39 and 40: Overall up-regulation of miRNAs and
Page 41 and 42: 41 · April 4, 2019 ARF6 signaling
Page 43 and 44: Network integrated analysis reveale
Page 45 and 46: Network integrated analysis reveale
Page 47 and 48: Conclusions ‣ 3DCoSeedsis TM are
Page 49 and 50: 49 · April 4, 2019 Thery et al., J
Page 51: Exosomes and Tumor Biology Group Ou

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