Oral Abstract Session 01 - Global HIV Vaccine Enterprise
Oral Abstract Session 01 - Global HIV Vaccine Enterprise
Oral Abstract Session 01 - Global HIV Vaccine Enterprise
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POSTERS<br />
Posters<br />
Topic 5: <strong>HIV</strong> Transmission and Viral Diversity<br />
P05.13<br />
<strong>HIV</strong>-1 Subtype B- and F1-Infected Subjects Display<br />
Higher Cross-Clade T-Cell Response Than Subtype<br />
C-Infected Subjects<br />
F.H. Côrtes 1 , G. Bello 1 , C. Vorsatz 2 , J.H. Pilotto 3 , B. Grinsztejn 4 ,<br />
V.G. Veloso 2 , A.R. Pinto 5 , M.G. Morgado 1<br />
1 Oswaldo Cruz Institute/FIOCRUZ, Rio de Janeiro, Brazil;<br />
2 Evandro Chagas Clinical Research Institute/FIOCRUZ, Rio<br />
de Janeiro, Brazil; 3 Nova Iguaçu General Hospital, Nova<br />
Iguaçu, Brazil; 4 Evandro Chagas Clinical Research Institute/<br />
FIOCRUZ, Rio de Janeiro, Brazil; 5 Department of Microbiology,<br />
Immunology and Parasitology/UFSC, Santa Catarina, Brazil<br />
Background: The impact of the extensive genetic diversity of the<br />
<strong>HIV</strong>-1 group M isolates and its implications for vaccine design<br />
have long been discussed. Studies indicate that Gag and Nef<br />
conserved epitopes are commonly recognized and give rise to<br />
high cross-clade responses. The aim of this study was to compare<br />
T-cell responses to peptide pools derivate from subtype B, C and<br />
F1 consensus, among Brazilian subjects infected with those three<br />
<strong>HIV</strong>-1 subtypes.<br />
Methods: The study included 32 subjects infected with <strong>HIV</strong>-1<br />
subtypes B (n=13), C (n=11) and F1 (n=8). Gag and Nef-specific<br />
T cell responses were evaluated by IFN-γ ELISpot assay, using<br />
peptide pools based on subtype B, C and F1 Brazilians consensus.<br />
Results: A high cross-clade response between subtypes B and<br />
F1 for both Gag and Nef regions was observed among <strong>HIV</strong>-1<br />
subtype B- and F1-infected subjects. We also found no significant<br />
difference in magnitude of responses between subtype B and C<br />
consensus peptides in subtype B-infected subjects.In contrast,<br />
the magnitude of T cell responses to consensus C peptides in<br />
Gag region was significantly higher than to consensus B peptides<br />
among <strong>HIV</strong>-1 subtype C-infected subjects. In Nef, subtype<br />
C-infected subjects showed higher T cell responses to C than to<br />
F1 consensus peptides. Moreover, subtype F1-infected subjects<br />
presented lower responses to subtype C peptides than to<br />
subtype F1 and B ones.<br />
Conclusion: Overall, the level of cross-clade response between<br />
subtypes B and F1 was higher than between subtype C and B or<br />
between subtype C and F1. Our data suggest that significance of<br />
the <strong>HIV</strong>-1 group M genetic diversity for vaccine design may be<br />
dependent of the subtypes involved.<br />
170<br />
AIDS <strong>Vaccine</strong> 2<strong>01</strong>2<br />
P05.14<br />
Emergence of Unique Recombinant Forms (URFs) in<br />
Indian <strong>HIV</strong>-1 Epidemic: Data from Nationwide Clinical<br />
Cohort Between 2007 and 2<strong>01</strong>1<br />
U. Neogi 1 , S. Gupta 1 , A. Shet 2 , A. De Costa 3 , R.L. Laishram 4 ,<br />
A. Wanchu 5 , V. Diwan 6 , A.C. Banerjea 7 , A. Sonnerborg 8<br />
1 St. John’s Medical College, Bangalore, India; 2 Department<br />
of Pediatrics, St. John’s Medical College Hospital, Bangalore,<br />
India; 3 Division of <strong>Global</strong> Health, Karolinska Institutet,<br />
Stockholm, Sweden; 4 Regional Institute of Medical Science,<br />
Imphal, India; 5 Department of Internal Medicine, PGIMER,<br />
Chandigarh, India; 6 Department of Public Health and<br />
Environment, R.D. Gardi Medical Colleg, Ujjain, India;<br />
7 National Institute of Immunology, New Delhi, India; 8 Divisions<br />
of Infectious Diseases, Department of Medicine Huddinge,<br />
Karolinska Institutet, Sweden<br />
Background: Current epidemiological studies in India have been<br />
limited to single or localized geographic settings within the<br />
country. In this study we aim to cherecterised the nationwide<br />
distribution pattern of the <strong>HIV</strong>-1 subtypes based on the data<br />
collected from clinical cohorts from 7 provinces from four regions<br />
in India (northern, north-eastern, central and southern)<br />
Methods: Blood samples were collected from 212 <strong>HIV</strong>-1<br />
seropositive subjects between 2007 and 2<strong>01</strong>1. <strong>HIV</strong>-1 subtypes<br />
were determined using at least two or three viral genes, gag, pol,<br />
and env using maximum likelihood tree. Recombination events<br />
were identified using RIP ver 3 tools followed by breakpoints<br />
analysis in Simplot version 3.5.1 and fragment-specific<br />
phylogenetic analysis.<br />
Results: When a single gene was used for subtype determination,<br />
the mean proportion of <strong>HIV</strong>-1C, B and A1 were 95.9%, 1.9%<br />
and 0.2% respectively while recombinants constituted 2.1%.<br />
The overall prevalence of URFs (BC/A1C) increased significantly<br />
to 10% when two (p
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