Detection of Enteric Bacteria in Raw Food Samples from Vietnam ...

More documents

Recommendations

Info

e.g. exempli gratia, for example et al et alia, and other people EAEC Enteroaggregative E. coli EDTA Ethylenediaminetetraacetic acid EIEC Enteroinvasive E. coli EHEC Enterohemorrhagic E. coli EMB Eosin methylene blue agar ENR Enrofloxacin EPEC Enteropathogenic E. coli EtBr Ethidium bromide ETEC Enterotoxigenic E. coli GEN Gentamicin g gram g relative centrifugal force h hour HCl Hydrochloride acid H2O Water HT High transducing Int407 Human embryonic intestine Kb Kilobase pairs KAN Kanamycin KIA Kligler Iron Agar L Litre LB-broth Luria Bertani Broth LB-agar Luria Bertani Agar xiii
LDC Lysine Decarboxylase MDR Multidrug resistant mg Milligram min Minutes NaCl Sodium chloride NAL Nalidixic acid NaOH Sodium hydroxide NCCLS National Committee for Clinical Laboratory Standards NCS Newborn calf serum NMKL Nordisk Metodikkommitté för Livsmedel, Nordic Committee on Food Analysis NOR Norfloxacin OD Optical density PBS Phosphate buffered saline PCR Polymerase chain reaction PFGE Pulsed field gel electrophoresis PMSF Phenylmethylsulfonyl fluoride pH negative logarithm to the base ten of the concentration of hydrogen ions rpm Revolutions per minute RFLP Restriction fragment length polymorphism s Seconds STR Streptomycin SUL Sulphafurazole TAE Tris, acetate, EDTA buffer TBE Tris-Borate-EDTA xiv
Page 1 and 2: Detection of Enteric Bacteria in Ra
Page 3 and 4: Acknowledgement I would first like
Page 5 and 6: Table of Content List of Abbreviati
Page 7 and 8: 2.1.6. Media and solutions for tiss
Page 9 and 10: Chapter 3: The Detection of Enteric
Page 11 and 12: 4.2.6. The transfer ability of anti
Page 13: Term Meaning AMP Ampicillin AMO Amo
Page 17 and 18: List of Figures Figure 1.1: The pos
Page 19 and 20: Figure 5.5: Agarose gel electrophor
Page 21 and 22: Table 4.2: E. coli isolates used to
Page 23 and 24: Abstract 1 ABSTRACT Food is essenti
Page 25 and 26: 3 ABSTRACT was also observed. In co
Page 27 and 28: 5 ABSTRACT The ownership of resista
Page 29 and 30: Chapter 1: Introduction 1.1. Enteri
Page 31 and 32: 9 CHAPTER 1: INTRODUCTION and about
Page 33 and 34: 11 CHAPTER 1: INTRODUCTION (20.7%),
Page 35 and 36: 13 CHAPTER 1: INTRODUCTION Typhoid
Page 37 and 38: 15 CHAPTER 1: INTRODUCTION proposed
Page 39 and 40: 17 CHAPTER 1: INTRODUCTION and oral
Page 41 and 42: 19 CHAPTER 1: INTRODUCTION the spec
Page 43 and 44: 21 CHAPTER 1: INTRODUCTION Unlike o
Page 45 and 46: 23 CHAPTER 1: INTRODUCTION is essen
Page 47 and 48: 25 CHAPTER 1: INTRODUCTION There ar
Page 49 and 50: 27 CHAPTER 1: INTRODUCTION Studies
Page 51 and 52: 29 CHAPTER 1: INTRODUCTION antibiot
Page 53 and 54: 31 CHAPTER 1: INTRODUCTION Stobberi
Page 55 and 56: 33 CHAPTER 1: INTRODUCTION The deve
Page 57 and 58: 35 CHAPTER 1: INTRODUCTION Plasmids
Page 59 and 60: Figure 1.2: General structures of i
Page 61 and 62: 39 CHAPTER 1: INTRODUCTION horizont
Page 63 and 64: 41 CHAPTER 1: INTRODUCTION 1998; Ri
Page 65 and 66:
43 CHAPTER 1: INTRODUCTION Salmonel
Page 67 and 68:
45 CHAPTER 1: INTRODUCTION Morabito
Page 69 and 70:
47 CHAPTER 2: GENERAL MATERIALS AND
Page 71 and 72:
Petri dish Nunc, Denmark 49 CHAPTER
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
2.1.3. General media and solutions
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Trypan blue solution: 1% (w/v) tryp
Page 85 and 86:
Page 87 and 88:
2.2.2.1.3. Isolation and identifica
Page 89 and 90:
Page 91 and 92:
Table 2.2: Bacterial strains and ba
Page 93 and 94:
2.2.3.2. Inoculation of test plates
Page 95 and 96:
2.2.4.3. Polymerase Chain Reaction
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
CHAPTER 3: THE DETECTION OF ENTERIC
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
CHAPTER 4: MOLECULAR GENETIC MECHAN
Page 163 and 164:
4.2. Method CHAPTER 4: MOLECULAR GE
Page 165 and 166:
Page 167 and 168:
No. CHAPTER 4: MOLECULAR GENETIC ME
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Isolate name Sources CHAPTER 4: MOL
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Isolates Sources CHAPTER 4: MOLECUL
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Integron size (kb CHAPTER 4: MOLECU
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
CHAPTER 5: PATHOGENICITY OF SALMONE
Page 243 and 244:
Page 245 and 246:
Page 247 and 248:
Primer set CHAPTER 5: PATHOGENICITY
Page 249 and 250:
Primer set CHAPTER 5: PATHOGENICITY
Page 251 and 252:
Page 253 and 254:
Page 255 and 256:
Page 257 and 258:
Page 259 and 260:
Page 261 and 262:
Page 263 and 264:
Page 265 and 266:
Isolate name CHAPTER 5: PATHOGENICI
Page 267 and 268:
Page 269 and 270:
Page 271 and 272:
Page 273 and 274:
Chapter 6: General Discussion 251 C
Page 275 and 276:
253 CHAPTER 6: GENERAL DISCUSSION s
Page 277 and 278:
255 CHAPTER 6: GENERAL DISCUSSION I
Page 279 and 280:
257 CHAPTER 6: GENERAL DISCUSSION m
Page 281 and 282:
259 CHAPTER 6: GENERAL DISCUSSION r
Page 283 and 284:
261 CHAPTER 6: GENERAL DISCUSSION p
Page 285 and 286:
263 REFERENCE Angkititrakul, S., C.
Page 287 and 288:
265 REFERENCE Bauernfeind, A., S. W
Page 289 and 290:
267 REFERENCE Boyd, D. A., G. A. Pe
Page 291 and 292:
269 REFERENCE Catry, B., H. Laevens
Page 293 and 294:
271 REFERENCE enterica serovars Typ
Page 295 and 296:
273 REFERENCE multiple-antibiotic r
Page 297 and 298:
275 REFERENCE Shiga toxin-producing
Page 299 and 300:
277 REFERENCE Fitzgerald, C., K. St
Page 301 and 302:
279 REFERENCE Graninger, W., K. Zed
Page 303 and 304:
281 REFERENCE Heffernan, E. J., J.
Page 305 and 306:
283 REFERENCE Jarrett, P., and M. S
Page 307 and 308:
285 REFERENCE Kauffmann, F. 1972. S
Page 309 and 310:
287 REFERENCE Leverstein-van Hall,
Page 311 and 312:
289 REFERENCE Maguire, H. C., A. A.
Page 313 and 314:
291 REFERENCE Meng, J., M. P. Doyle
Page 315 and 316:
293 REFERENCE Nakamura, M., S. Sato
Page 317 and 318:
295 REFERENCE Christenson, M. L. Bi
Page 319 and 320:
297 REFERENCE Phan, Q., P. Mshar, T
Page 321 and 322:
299 REFERENCE Refsum, T., E. Heir,
Page 323 and 324:
301 REFERENCE Salyers, A. A. 1993.
Page 325 and 326:
303 REFERENCE Identification of ant
Page 327 and 328:
305 REFERENCE patterns produced by
Page 329 and 330:
307 REFERENCE Vaudaus, P., and F. A
Page 331 and 332:
309 REFERENCE White, D. G., S. Zhao
Page 333 and 334:
311 REFERENCE Yavzori, M., N. Porat
Page 335 and 336:
APPENDIX Table A1: Salmonella spp.
Page 337 and 338:
Isolates Source Group Serotype 315
Page 339 and 340:
Table A2: E. coli isolates which we
Page 341 and 342:
Isolates Source AMP AMO AUG TET 319
Page 343 and 344:
Table A3: V. parahaemolyticus isola
Page 345:
323 CONFERENCES/ PROCEEDINGS and PU
show all

Detection of Enteric Bacteria in Raw Food Samples from Vietnam ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?