Detection of Enteric Bacteria in Raw Food Samples from Vietnam ...

Figure 4.8: RFLP of 2.65 kb class 1 integron PCR product of isolates E/C/16a and
E/C/21a. ............................................................................................................... 191
Figure 4.9: Agarose gel electrophoresis of PCRs amplification of SGI1 of isolate
S/C/21a. ............................................................................................................... 199
Figure 4.10: Agarose gel electrophoresis of PCRs amplification of SGI1 of isolate
DT104. ................................................................................................................. 199
Figure 4.11: Southern blot hybridisation of DT104 (lane 1) and S. Albany (lane 2) by
probing with aadA2 gene. .................................................................................... 200
Figure 4.12: Agarose gel electrophoresis of PCRs amplification of DE region of isolate
S/C/21a (lane 1) and DT104 (lane 2). .................................................................. 201
Figure 4.13: RFLP of PCR product of DE regions with enzyme DraIII of isolate
S/C/21a (lane 1) and DT104 (lane 2) ................................................................... 202
Figure 4.14: RFLP of PCR product of DE regions with enzyme PstI and BamHI of
isolate S/C/21a (lane 1, 3 respectively) and DT104 (lane 2, 4 respectively) ....... 202
Figure 4.15: Macrorestriction analysis by PFGE of genomic DNA digested by XbaI of
DT104 (lane 1) and S. Albany S/C/21a (lane 2). ................................................. 205
Figure 4.16: Plasmid DNA of donor S/C/5 (lane 1), recipient E/P/8a (lane 2), and
transconjugant ...................................................................................................... 211
Figure 4.17: The transfer of plasmids DNA in conjugation experiment. .............................. 212
Figure 5.1: Amplification bands on 2% agarose gel corresponding to all 18 PCR sets I-
XVIII for the detection of 58 virulence genes ..................................................... 229
Figure 5.2: PCR products of the invA gene of Salmonella spp. on 1.5% agarose gel
electrophoresis ..................................................................................................... 231
Figure 5.3: PCR products of the spvC gene of Salmonella spp. on 1.5% agarose gel
electrophoresis ..................................................................................................... 233
Figure 5.4: In vitro invasion assay with INT-407 cells. ........................................................ 235
xvii