Transplantation Immunology.pdf - E-Lib FK UWKS

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M E T H O D S I N M O L E C U L A R B I O L O G Y Transplantation Immunology Methods and Protocols Edited by Philip Hornick National Heart and Lung Institute, London, UK Marlene Rose National Heart and Lung Institute, Harefield, UK
© 2006 Humana Press Inc. 999 Riverview Drive, Suite 208 Totowa, New Jersey 07512 www.humanapress.com All rights reserved. No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise without written permission from the Publisher. Methods in Molecular BiologyTM is a trademark of The Humana Press Inc. All papers, comments, opinions, conclusions, or recommendations are those of the author(s), and do not necessarily reflect the views of the publisher. This publication is printed on acid-free paper. ∞ ANSI Z39.48-1984 (American Standards Institute) Permanence of Paper for Printed Library Materials. Cover illustration: From Fig. 3, Chapter 17, “Experimental Models of Graft Arteriosclerosis,” by Bezhad Soleimani and Victor C. Shi. Production Editor: Robin B. Weisberg Cover design by Patricia F. Cleary For additional copies, pricing for bulk purchases, and/or information about other Humana titles, contact Humana at the above address or at any of the following numbers: Tel.: 973-256-1699; Fax: 973-256-8341; E-mail: orders@humanapr.com; or visit our Website: www.humanapress.com Photocopy Authorization Policy: Authorization to photocopy items for internal or personal use, or the internal or personal use of specific clients, is granted by Humana Press Inc., provided that the base fee of US $30.00 per copy is paid directly to the Copyright Clearance Center at 222 Rosewood Drive, Danvers, MA 01923. For those organizations that have been granted a photocopy license from the CCC, a separate system of payment has been arranged and is acceptable to Humana Press Inc. The fee code for users of the Transactional Reporting Service is: [1-58829-544-3/06 $30.00]. Printed in the United States of America. 10 9 8 7 6 5 4 3 2 1 eISBN: 1-59745-049-9 ISSN: 1064-3745 Library of Congress Cataloging-in-Publication Data Transplantation immunology : methods and protocols / edited by Philip Hornick, Marlene Rose. p. ; cm. -- (Methods in molecular biology, ISSN 1064-3745 ; v. 333) Includes bibliographical references and index. ISBN 1-58829-544-3 (alk. paper) 1. Transplantation immunology. I. Hornick, Philip. II. Rose, Marlene L. III. Series: Methods in molecular biology (Clifton, N.J.) ; v. 333. [DNLM: 1. Graft Rejection--diagnosis. 2. Graft Rejection--immunology. 3. Laboratory Techniques and Procedures. 4. Organ Transplantation --adverse effects. 5. Transplantation, Homologous--immunology. W1 ME9616J v.333 2006 / WO 680 T7725 2006] QR188.8T732 2006 617.9'5--dc22 2005028830
Page 1 and 2: METHODS IN MOLECULAR BIOLOGY 333 T
Page 3: M E T H O D S I N M O L E C U L A R
Page 8 and 9: Contents Preface ..................
Page 10 and 11: Contributors PAUL J. R. BARTON •
Page 12 and 13: Current Status of Renal Transplanta
Page 38: Current Status of Renal Transplanta
Page 41 and 42: METHODS IN MOLECULAR BIOLOGY 333 T
Page 43 and 44: 30 Friend and Imber meaningful surv
Page 45 and 46: 32 Friend and Imber the most urgent
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Page 49 and 50: 36 Friend and Imber inotrope use, c
Page 51 and 52: 38 Friend and Imber very experience
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42 Friend and Imber subsequent isol
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44 Friend and Imber 21. Heffron, T.
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46 Friend and Imber 53. Saudubray,
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48 Lakey et al. There are an estima
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50 Lakey et al. diabetic to burn su
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52 Lakey et al. ber 31, 2000, less
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54 Lakey et al. Fig. 1. Schematic v
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56 Lakey et al. Table 1 Effect of I
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58 Lakey et al. from suitable donor
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60 Lakey et al. mined by high-perfo
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62 Lakey et al. Fig. 3. (A) Mean po
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64 Lakey et al. Fig. 5. HbA1c at 3-
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66 Lakey et al. All patients enjoy
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68 Lakey et al. 6. Sirolimus-based
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70 Lakey et al. Fig. 7. Two-layer (
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72 Table 2 Human Islet Transplantat
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74 Lakey et al. 3.4. Single-Donor I
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76 Lakey et al. difficult to isolat
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78 Lakey et al. 4.2. Islet Transpla
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80 Lakey et al. Table 3 Major Targe
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82 Lakey et al. CD40L (anti-CD154)
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84 Lakey et al. as kidney, heart, o
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86 Lakey et al. 14. Despres, J. P.,
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88 Lakey et al. tion despite a weak
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90 Lakey et al. 77. Maffi, P., Bert
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92 Lakey et al. 108. Idezuki, Y., G
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94 Lakey et al. 138. http://www.imm
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96 Lakey et al. tory reaction trigg
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98 Lakey et al. 198. O’Neil, J. J
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100 Lakey et al. 232. Delves, P. J.
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102 Lakey et al. 259. Grewal, I. S.
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Status of Lung Transplantation 105
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Chronic Rejection in the Heart 131
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Direct and Indirect Allorecognition
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HLA Typing and Organ Transplantatio
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Gene Transfer to Solid Organs 175 8
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Gene Transfer to Solid Organs 177 t
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Gene Transfer to Solid Organs 181
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183 Adenovirs (Ad) Nonenveloped vir
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Gene Transfer to Solid Organs 185 t
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Gene Transfer to Solid Organs 187 A
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Gene Transfer to Solid Organs 189 c
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202 Collins Table 1 Advantages and
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204 Collins The advantage of these
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206 Collins have used liposomal del
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208 Table 2 Ligands Used for Recept
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210 Collins It must not be forgotte
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212 Collins Fig. 3. Receptor-mediat
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214 Collins Receptor-mediated polyl
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216 Collins there are, to date, mor
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218 Collins 29. Niculescu-Duvaz, D.
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220 Collins 59. Wolfert, M. A. and
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222 Collins 91. Simoes, S., Slepush
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224 Collins 122. Hart, S. L., Harbo
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Antibody Relevance After Transplant
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248 Graca and Waldmann 2. The Use o
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250 Graca and Waldmann Fig. 1. Indu
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252 Graca and Waldmann Despite this
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254 Graca and Waldmann transforming
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256 Graca and Waldmann The role of
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258 Graca and Waldmann Fig. 5. A re
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260 Graca and Waldmann 4. Kahan, B.
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262 Graca and Waldmann 35. Powrie,
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264 Graca and Waldmann 67. Annacker
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266 Graca and Waldmann 96. Bommired
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Immune Monitoring in Transplantatio
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283 Table 1 Current and Potential F
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Proteomics and Laser Microdissectio
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Real-Time PCR 305 14 Real-Time Quan
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Real-Time PCR 307 Table 1 Real-Time
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Real-Time PCR 309 Fig. 2. Determina
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Real-Time PCR 311 such as Applied B
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Real-Time PCR 313 Table 2 Compariso
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315 Fig. 4. Quantification of RNA u
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Real-Time PCR 317 correlate directl
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319 Table 4 Common Internal Control
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321 Table 5 Examples of Single-Nucl
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Real-Time PCR 323 3.2. Real-Time PC
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Real-Time PCR 325 3.3.2. Sources of
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Real-Time PCR 327 9. Barton, P. J.
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Real-Time PCR 329 40. Primer expres
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Organ Preservation 331 15 Organ Pre
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Organ Preservation 333 2. Managemen
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Organ Preservation 335 tal observat
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Organ Preservation 337 donors, depe
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Organ Preservation 339 Table 1 Lung
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Organ Preservation 341 obtained wit
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Organ Preservation 343 Fig. 2. Acti
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Organ Preservation 345 Fig. 3. Outl
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Organ Preservation 347 and related
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Organ Preservation 349 mic, ischemi
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Organ Preservation 351 While these
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Organ Preservation 353 experimental
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Organ Preservation 355 with inhaled
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Organ Preservation 357 cellular sol
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Organ Preservation 359 4. Rosengard
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Organ Preservation 361 35. Powner,
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Organ Preservation 363 64. Hendry,
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Organ Preservation 365 factor-media
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Organ Preservation 367 120. Bonser,
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Organ Preservation 369 150. Macdona
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Organ Preservation 371 180. Carbogn
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209. Szabo, G., Soos, P., Bahrle, S
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376 Hopkins the extent of cold isch
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378 Hopkins or sequential administr
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380 Hopkins Table 2 Current Availab
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382 Hopkins 2. Renal failure: acute
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384 Table 4 Comparison of Azathiopr
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386 Hopkins Table 6 Side-Effect Pro
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388 Hopkins baseline immunosuppress
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390 Hopkins supplement to maintenan
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392 Hopkins 3. Kauppinen, H., Soots
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394 Hopkins uline versus no inducti
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396 Hopkins 62. Mycophenolate Mofet
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398 Hopkins 89. Mentzer, R. M., Jah
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Experimental Models of Graft Arteri
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405 Fig. 1. Variation in GA severit
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