3130/3130xl Genetic Analyzers Getting Started Guide, Rev - Applied ...
3130/3130xl Genetic Analyzers Getting Started Guide, Rev - Applied ...
3130/3130xl Genetic Analyzers Getting Started Guide, Rev - Applied ...
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Notes<br />
Chapter 1 Preparing the Instrument<br />
Application Summary Tables<br />
Fragment<br />
Analysis<br />
Resolution<br />
Performance and<br />
Specifications<br />
Run Modules<br />
Decide what combination of capillary array and polymer from the table below matches<br />
your resolution and performance specifications. See “Fragment Analysis Kits and Run<br />
Modules” on page 5 for information on capillary, polymer, and run module.<br />
Capillary<br />
Length<br />
(cm)<br />
Polymer<br />
Type<br />
High Throughput, Small Size Fragment Analysis<br />
FragmentAnalysis22<br />
_POP4<br />
Run<br />
Time<br />
(min)<br />
24 hr Throughput (GT a )<br />
<strong>3130</strong><br />
Analyzer<br />
<strong>3130</strong>xl<br />
Analyzer<br />
Resolution<br />
(bp)<br />
Specification<br />
(SD) b<br />
22 POP-4 20 5,760 23,040 250 0.15<br />
SNP22_POP4 22 20 5,760 23,040 120 0.50<br />
Standard Fragment Analysis<br />
FragmentAnalysis36<br />
_POP4<br />
HIDFragmentAnalysis<br />
36_POP4<br />
36 POP-4 45 2,560 10,240 500 0.15<br />
45 2,560 10,240 500 0.15<br />
SNP36_POP4 30 3,840 15,360 120 0.50<br />
FragmentAnalysis36<br />
_POP7<br />
FragmentAnalysis50<br />
_POP4<br />
FragmentAnalysis50<br />
_POP6<br />
FragmentAnalysis50<br />
_POP7<br />
a 20 GT (Genotypes)/capillary/injection.<br />
b 1bp resolution at 99.99% accuracy.<br />
POP-7 35 3,290 13,170 500 0.15<br />
50 POP-4 65 1,760 7,040 500 0.15<br />
POP-6 90 1,200 4,800 500 0.15<br />
POP-7 50 2,300 9,220 500 0.15<br />
4 <strong>Applied</strong> Biosystems <strong>3130</strong>/<strong>3130</strong>xl <strong>Genetic</strong> <strong>Analyzers</strong> <strong>Getting</strong> <strong>Started</strong> <strong>Guide</strong><br />
DRAFT<br />
November 3, 2004 9:51 am, 01_PrepInstrument.fm
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