3130/3130xl Genetic Analyzers Getting Started Guide, Rev - Applied ...
3130/3130xl Genetic Analyzers Getting Started Guide, Rev - Applied ...
3130/3130xl Genetic Analyzers Getting Started Guide, Rev - Applied ...
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Notes<br />
Chapter 1 Preparing the Instrument<br />
Preparing Buffer and Filling Reservoirs<br />
Preparing Buffer and Filling Reservoirs<br />
Required Materials<br />
CHEMICAL HAZARD. 10X<br />
<strong>Genetic</strong> Analyzer Buffer with EDTA may cause<br />
eye, skin, and respiratory tract irritation. Read the<br />
MSDS, and follow the handling instructions. Wear<br />
appropriate protective eyewear, clothing, and gloves.<br />
Required materials to prepare 1X running buffer:<br />
10X <strong>Genetic</strong> Analyzer Buffer (P/N 402824)<br />
Purified (distilled or deionized) water<br />
50 mL graduated cylinder<br />
Preparing Buffer for a Single Run<br />
1. Add 5 mL of 10X <strong>Genetic</strong> Analyzer buffer into a<br />
graduated cylinder.<br />
2. Add purified water to bring the total volume up<br />
to 50 mL.<br />
3. Mix well.<br />
Storing Buffer<br />
Store 1X running buffer at:<br />
2 to 8 °C for up to 1 month<br />
Room temperature for 1 week<br />
16 <strong>Applied</strong> Biosystems <strong>3130</strong>/<strong>3130</strong>xl <strong>Genetic</strong> <strong>Analyzers</strong> <strong>Getting</strong> <strong>Started</strong> <strong>Guide</strong><br />
50 mL<br />
total<br />
50 mL graduated cylinder<br />
Buffer Storage Conditions<br />
Option A Option B<br />
2 °C to<br />
8 °C<br />
Su M T W Th F S<br />
1 month<br />
20 °C to<br />
25 °C<br />
Su M T W Th F S<br />
7 days<br />
45 mL DI H 2O<br />
5 mL 10X <strong>Genetic</strong><br />
Analyzer buffer<br />
DRAFT<br />
November 3, 2004 9:51 am, 01_PrepInstrument.fm
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