3130/3130xl Genetic Analyzers Getting Started Guide, Rev - Applied ...

Chapter 1 Preparing the Instrument
Preparing the Instrument
When to Replenish or Change Polymer
IMPORTANT! Always replace
polymer that has been on the
instrument longer than one week.
Before Using the Polymer
1. Remove the polymer from 4 °C storage.
2. Loosen the cap and bring the polymer to room
temperature.
3. To dissolve deposits, tighten the cap and gently
swirl the polymer.
Replenishing the Polymer
IMPORTANT! Wear gloves while
handling polymer, the capillary array,
septa, or buffer reservoirs.
Notes
Su M T W Th F S
If polymer on the instrument... Then ...
has been on less than one week and is in sufficient quantity
to complete your runs a
has been on less than one week, and insufficient in quantity
to complete your runs
has been on longer than one week
is the wrong type (a change between POP-4, POP-6, and/or
POP-7 polymers is required)
CHEMICAL HAZARD. POP
polymer may cause eye, skin, and respiratory tract
irritation. Please read the MSDS, and follow the
handling instructions. Wear appropriate protective
eyewear, clothing, and gloves. Use for research and
development purposes only.
1. Click < Instrument Name> in the tree pane.
Remove all bubbles, and then proceed with instrument
preparation.
Add fresh polymer to the polymer supply by following the
Replenish Polymer Wizard.
Replace the installed polymer type with a different type by
following the Change Polymer Type Wizard.
a A 3130xl genetic analyzer run uses 50 to 80 µL of polymer and a 3130 genetic analyzer run uses ~25 to 40µL of polymer.
14 Applied Biosystems 3130/3130xl Genetic Analyzers Getting Started Guide
DRAFT
November 3, 2004 9:51 am, 01_PrepInstrument.fm