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3130/3130xl Genetic Analyzers Getting Started Guide, Rev - Applied ...

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Notes<br />

Chapter 2 Performing a Spatial Calibration<br />

Spatial Calibration<br />

Spatial Calibration<br />

When to Perform<br />

the Calibration<br />

The <strong>3130</strong>/<strong>3130</strong>xl genetic analyzer Data Collection software uses images collected during<br />

the spatial calibration to establish a relationship between the signal emitted by each<br />

capillary and the position where that signal falls on and is detected by the CCD camera.<br />

You are required to perform a spatial calibration when you:<br />

Install or replace a capillary array<br />

Temporarily remove the capillary array from the detection block<br />

Move the instrument<br />

Creating a Spatial Calibration File<br />

Positions (pixel)<br />

of each capillary<br />

1. In the tree pane of the Data Collection software, click<br />

GA Instruments > ga<strong>3130</strong> or ga<strong>3130</strong>xl > instrument<br />

name > Spatial Run Scheduler.<br />

Spatial<br />

profile<br />

Start, Accept<br />

or Reject<br />

Spatial profile<br />

22 <strong>Applied</strong> Biosystems <strong>3130</strong>/<strong>3130</strong>xl <strong>Genetic</strong> <strong>Analyzers</strong> <strong>Getting</strong> <strong>Started</strong> <strong>Guide</strong><br />

DRAFT<br />

November 3, 2004 9:51 am, 02_SpatialCal.fm

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