Epidemiología de Plum pox virus y Citrus tristeza virus en bloques ...

plants analyzed being spring and summer the best times for detection of PPV followed
by autumn and it is not recommended use of compounds in winter.
Latent models have been applied for first time in plant pathology for estimating
the different diagnostic parameters of Spot real-time RT-PCR and ELISA-DASI
techniques. It has been shown that they can be applied to the estimation of the
parameters for the diagnosis of new and existing validated techniques for detection of
plant pathogens.
As a general conclusion, this Thesis provides a basic understanding of the
epidemiology of sharka and tristeza diseases in nursery blocks, which may be applied to
the design of control strategies. Furthermore, it suggests the use of environmentally
friendly control methods with zero residues such as the employment of less susceptible
or resistant PPV Prunus rootstocks and the use of mineral oils. Also, the molecular
techniques Spot real-time and Tissue-print RT-PCR have been validated for a reliable
and accurate detection of PPV and CTV in nursery plants. The use of both these
techniques provides a better guarantee for the virus-free status of analyzed plant
material than other techniques employed so far.
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