2004 - Instituto de Biotecnología - UNAM

procariotes y de células de eucariotes inferiores y superiores. Destacan en esta área el desarrollo de los
bioprocesos para la producción de dos proteínas humanas recombinantes: la insulina y la hormona de
crecimiento.
PUBLICACIONES 2004
Cortés G, Trujillo M, Ramírez O, Galindo E . 2004. Production of B-galactosidase by Kluyveromyces
marxiamus under oscillating dissolved oxygen tension. Process Biochem, 40 , 773-778.
Estrada-Mondaca S, Delgado-Bustos LA, Ramírez OT . Mannosamine supplementation extends Nacetylglucosaminylation
of r-human-SeAP produced in Trichoplusia in insect cell culture. Biotechnol Appl
Biochem [Disponible en línea Dec, 2004].
Palomares LA, López S, Ramírez OT . 2004. Utilization of oxygen uptake rate to assess the role of
glucose and glutamine in the metabolism of infected insect cell cultures. Biochem Eng J, 19 , 87-93.
Palomares LA, Estrada-Mondaca S, Ramírez OT . 2004. Production of recombinant proteins:
challenges and solutions. Methods Mol Biol, 267 , 15-52.
Serrato JA, Palomares LA, Meneses-Acosta A, Ramírez OT . 2004. Heterogeneous conditions in
dissolved oxygen affect N-glycosylation but not productivity of a monoclonal antibody in hybridoma
cultures. Biotechnol Bioeng, 88 , 176-188.
PUBLICACIONES SELECTAS
Mena JA, Ramírez OT, Palomares LA . 2003. Titration of non-occluded baculovirus using a cell viability
assay. Biotechniques, 34, 260-264.
Palomares L, López-Charretón S, Ramírez O . 2002. Strategies for manipulating the relative
concentration of recombinant rotavirus structural proteins during simultaneous production by insect cells.
Biotechnol Bioeng, 78 , 635-644.
Meneses-Acosta A, Mendonca R, Merchant H, Covarrubias L, Ramírez O . 2001. Comparative
characterization of cell death between Sf9 insect cells and hybridoma cultures. Biotechnol Bioeng , 72,
324-331.
Higadera A, Possani LD, Ramírez, O . 1997. The use of culture redox potential and oxygen uptake rate
fos assessing glucose and glutamine depletion in hybridoma cultures. Biotechnol Bioeng, 56 , 555-563.
Ramírez O, Zamora R, Quintero R, López-Munguía A . 1994. Exponentially fed-batch cultures as an
alternative to chemostats: the case of penicillin acylase production by recombinant E. coli . Enzyme
Microb Technol, 16 , 895-903.
Fuentes de financiamiento: CONACyT (20401); DGAPA/UNAM (IN218202), (IN220503), (IX254404).